Section of Pharmacology and Clinical Pharmacology, Faculty of Medicine, University Hospital, Trakia University, Stara Zagora, Bulgaria.
Section of Dermatovenereology, Clinic of Dermatology and Venereology, Faculty of Medicine, University Hospital, Trakia University, Stara Zagora, Bulgaria.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2024 Jun;168(2):124-131. doi: 10.5507/bp.2023.043. Epub 2023 Nov 14.
The present study aimed to assess vitamin D status and serum concentrations of pro-inflammatory cytokines IL-17, Il-23, and IL-18 in patients with chronic plaque psoriasis and their association with various demographic and clinical characteristics.
The study was conducted during the autumn/winter period on 48 patients with chronic plaque psoriasis and 48 controls. Total serum 25(OH)D level was determined with Roche Elecsys 2010 Vitamin D total assay. Commercial ELISA kits were used for quantifying the serum levels of IL-17A, IL-18, and IL-23.
Serum 25(OH)D had a median value of 16.95 ng/mL (IQR 10.8-23.50) for patients with psoriasis and 18.80 ng/mL (IQR 15.45-25.85) for the control group (P=0.09). A moderate negative correlation was found between PASI score and 25(OH)D levels (r=-0.34; P=0.02). The serum levels of IL-17 (P=0.001), IL-23 (P=0.01) and IL-18 (P=0.02) were significantly higher in the patient group compared to controls. IL-17 concentrations were higher in patients with moderate to severe psoriasis compared to patients with mild psoriasis (P=0.003). No significant correlations were detected between the serum concentrations of 25(ОH)D and IL-17, IL-23, and IL-18.
It was confirmed that IL-17 serum level is associated with psoriasis severity. Measurement of 25(OH)D serum concentration can be useful in patients with moderate to severe psoriasis with or without comorbidities. A direct association between 25(OH)D serum concentration and the serum concentrations of IL-17, IL-23, or IL-18 was not identified in this study.
本研究旨在评估慢性斑块型银屑病患者的维生素 D 状态和促炎细胞因子白细胞介素-17（IL-17）、白细胞介素-23（IL-23）和白细胞介素-18（IL-18）的血清浓度，并探讨其与各种人口统计学和临床特征的关系。
本研究于秋冬季节在 48 例慢性斑块型银屑病患者和 48 例对照者中进行。采用罗氏 Elecsys 2010 维生素 D 总检测试剂盒测定总血清 25（OH）D 水平。采用商业 ELISA 试剂盒定量检测血清中 IL-17A、IL-18 和 IL-23 的水平。
银屑病患者的血清 25（OH）D 中位数为 16.95ng/mL（IQR 10.8-23.50），对照组为 18.80ng/mL（IQR 15.45-25.85）（P=0.09）。PASI 评分与 25（OH）D 水平呈中度负相关（r=-0.34；P=0.02）。与对照组相比，患者组的血清 IL-17（P=0.001）、IL-23（P=0.01）和 IL-18（P=0.02）水平显著升高。与轻度银屑病患者相比，中重度银屑病患者的血清 IL-17 浓度更高（P=0.003）。本研究未发现血清 25（OH）D 浓度与 IL-17、IL-23 和 IL-18 之间存在显著相关性。
本研究证实了血清 IL-17 水平与银屑病严重程度相关。在合并或不合并合并症的中重度银屑病患者中，测量血清 25（OH）D 浓度可能是有用的。在本研究中，未发现血清 25（OH）D 浓度与血清 IL-17、IL-23 或 IL-18 浓度之间存在直接关联。
