Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences (TUMS), Tehran, Iran.
Department of Dental Biomaterials, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
ScientificWorldJournal. 2023 Nov 6;2023:7532898. doi: 10.1155/2023/7532898. eCollection 2023.
This study aimed to assess the effect of addition of fluorohydroxyapatite (FHI) on biological and physical properties of mineral trioxide aggregate (MTA) Angelus.
In this in vitro, experimental study, nano-FHI powder was first synthesized, and the morphology and chemical structure of particles were evaluated by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). Three groups were evaluated in this study: MTA Angelus, MTA modified with 10% FHA, and MTA modified with 15% FHA. After mixing, the materials were applied to ring molds (10 mm diameter, 1 mm height), and the setting time of the three groups was evaluated according to ISO6876 and ASTMC266-03 with a Gillmore needle. The pH was measured using a pH meter at 24 and 48 hours and 7 days after mixing. The cytotoxicity of the materials was assessed in freshly mixed form and after 1 and 7 days using the methyl thiazolyl tetrazolium (MTT) assay according to ISO10993-5. Data were analyzed by one-way and repeated measures ANOVA and Tukey's test (alpha = 0.05).
The addition of FHA to MTA significantly decreased the initial setting time ( < 0.05) and had no significant effect on cell viability (compared with pure MTA Angelus) at 1 and 7 days. However, modified MTA groups in freshly mixed form showed significantly lower cell viability ( < 0.05). The pH remained alkaline at all time points.
Addition of 15% FHA to MTA Angelus decreased its setting time with no adverse effect on cell viability (except for fresh form) or pH.
本研究旨在评估氟羟磷灰石（FHA）的添加对 Angelus 矿化三氧化物聚合体（MTA）的生物学和物理性能的影响。
在这项体外实验研究中，首先合成纳米 FHA 粉末，通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）和 X 射线衍射（XRD）评估颗粒的形态和化学结构。本研究评估了三组材料：MTA Angelus、添加 10% FHA 的 MTA 和添加 15% FHA 的 MTA。混合后，将材料涂敷到环模（直径 10mm，高度 1mm）中，根据 ISO6876 和 ASTMC266-03 用 Gillmore 针评估三组的凝固时间。混合后 24、48 小时和 7 天时使用 pH 计测量 pH 值。根据 ISO10993-5 用甲基噻唑基四唑（MTT）测定法评估新鲜混合和混合 1 和 7 天后材料的细胞毒性。数据采用单因素和重复测量方差分析及 Tukey 检验进行分析（α=0.05）。
FHA 的添加显著降低了 MTA 的初凝时间（<0.05），但在第 1 和第 7 天对细胞活力（与纯 MTA Angelus 相比）没有显著影响。然而，新鲜形式的改性 MTA 组的细胞活力明显较低（<0.05）。所有时间点 pH 值均保持碱性。
在 MTA Angelus 中添加 15% FHA 可缩短其凝固时间，且对细胞活力（新鲜形式除外）或 pH 值无不良影响。
