Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences (TUMS), Tehran, Iran.
Department of Endodontics, Tehran University of Medical Sciences (TUMS), International Campus, Tehran, Iran.
ScientificWorldJournal. 2021 Nov 24;2021:6318690. doi: 10.1155/2021/6318690. eCollection 2021.
is the dominant microorganism in chronic apical periodontitis. It is more resistant to local antiseptic agents than other endodontic microorganisms. Currently, mineral trioxide aggregate (MTA) is considered as an ideal material in many endodontic procedures. Some studies have shown that MTA has good antibacterial activity against . However, some studies have investigated the effect of incorporating some materials into MTA on its antibacterial activity against . No study has evaluated the effect of incorporating fluorohydroxyapatite nanoparticles (nano-FHA) on the antimicrobial activity of MTA. Therefore, the present study evaluated the antimicrobial effect of MTA mixed with nano-FHA on in vitro. The study was carried out on 18 samples in three groups: pure MTA, MTA mixed with 10 wt% of nano-FHA, and MTA mixed with 15 wt% of nan-FHA. The effect of nano-FHA on the antibacterial activity of MTA on was evaluated by evaluating the growth inhibition zone around each sample. The antimicrobial effect of samples on inhibiting biofilm formation and inhibiting microbial growth of in the planktonic phase was evaluated by disk agar diffusion (DAD), biofilm inhibition assay (BIA), and direct contact assay (DCA) tests, respectively. All the above tests were analyzed after 24 and 72 hours. Factorial designs were used for statistical analyses. Tukey tests were used for two-by-two comparisons. All the statistical analyses were carried out with SPSS 26. DAD results showed no formation of the growth inhibition zone in all the samples after 24 and 72 hours. The microbial colony counts in the BIA and DCA tests in the groups modified with FHA nanoparticles were significantly lower than the pure MTA group ( < 0.05). The microbial colony counts increased in all the groups over time ( < 0.05). Incorporating nano-FHA into MTA improved the antimicrobial activity of MTA against compared to pure MTA. The highest antimicrobial activity was achieved after incorporating 15 wt% of nano-FHA into MTA at the 72-hour interval.
是慢性根尖周炎的优势微生物。它比其他牙髓微生物更能抵抗局部防腐剂。目前，三氧化矿物聚合体（MTA）被认为是许多牙髓治疗过程中的理想材料。一些研究表明，MTA 对 具有良好的抗菌活性。然而，一些研究调查了将某些材料掺入 MTA 对其抗菌活性对 的影响。没有研究评估将氟羟基磷灰石纳米粒子（nano-FHA）掺入 MTA 对 MTA 抗菌活性的影响。因此，本研究评估了 MTA 与纳米 FHA 混合物对 体外的抗菌效果。该研究在三组共 18 个样本中进行：纯 MTA、MTA 混合 10wt%纳米 FHA 和 MTA 混合 15wt%纳米 FHA。通过评估每个样本周围的生长抑制区来评估纳米 FHA 对 MTA 对 的抗菌活性的影响。通过圆盘琼脂扩散（DAD）试验、生物膜抑制试验（BIA）和直接接触试验（DCA）分别评估样品对抑制 生物膜形成和抑制浮游期 微生物生长的抗菌效果。所有上述测试均在 24 小时和 72 小时后进行分析。采用析因设计进行统计分析。Tukey 检验用于两两比较。所有统计分析均采用 SPSS 26 进行。DAD 结果显示，所有样本在 24 小时和 72 小时后均未形成生长抑制区。在含有 FHA 纳米粒子的改性组中，BIA 和 DCA 试验中的微生物菌落计数明显低于纯 MTA 组（ < 0.05）。所有组的微生物菌落计数随时间增加（ < 0.05）。与纯 MTA 相比，将纳米 FHA 掺入 MTA 可提高 MTA 对 的抗菌活性。在 72 小时间隔内，将 15wt%的纳米 FHA 掺入 MTA 可获得最高的抗菌活性。
