Ramsay G, Stanton L, Schwab M, Bishop J M
Mol Cell Biol. 1986 Dec;6(12):4450-7. doi: 10.1128/mcb.6.12.4450-4457.1986.
N-myc is a gene whose amplification has been implicated in the genesis of several malignant human tumors. We have identified two proteins with molecular weights of 65,000 and 67,000 encoded by N-myc. The abundance of these proteins in tumor cells was consonant with the extent of amplification of N-myc. The two proteins apparently arose from the same mRNA, were phosphorylated, were exceptionally unstable, were located in the nucleus of cells, and bound to both single- and double-stranded DNA. These properties suggest that the products of N-myc and of the related proto-oncogene c-myc may have similar biochemical functions and that N-myc may be a regulatory gene. Our findings sustain the view that inordinate expression of N-myc may contribute to the genesis of several different human tumors.
N - myc是一种其扩增与多种人类恶性肿瘤发生有关的基因。我们已鉴定出由N - myc编码的两种分子量分别为65,000和67,000的蛋白质。这些蛋白质在肿瘤细胞中的丰度与N - myc的扩增程度一致。这两种蛋白质显然来自同一mRNA，被磷酸化，异常不稳定，位于细胞核中，并与单链和双链DNA结合。这些特性表明N - myc和相关原癌基因c - myc的产物可能具有相似的生化功能，并且N - myc可能是一个调控基因。我们的研究结果支持这样一种观点，即N - myc的过度表达可能促成几种不同人类肿瘤的发生。
