Weller P A, Price M, Isenberg H, Edwards Y H, Jeffreys A J
Mol Cell Biol. 1986 Dec;6(12):4539-47. doi: 10.1128/mcb.6.12.4539-4547.1986.
We showed that myoglobin gene transcription and the appearance of myoglobin occur very early in myogenesis, in both humans and mice. In contrast to the contractile protein genes, there is a subsequent increase of 50- to 100-fold in myoglobin mRNA and protein levels during later muscle development. Myoglobin and myoglobin mRNA are present at elevated levels in fetal heart and are also detectable at low levels in adult smooth muscle. The absolute level of myoglobin mRNA in highly myoglobinized seal muscle is very high [2.8% of the total population of poly(A)+ RNAs]. Levels of myoglobin in seal skeletal muscle and in various human muscle types appear to be determined by the size of the myoglobin mRNA pool. In contrast, low levels of myoglobin in mouse skeletal muscle are not apparently correlated with low levels of myoglobin mRNA. As expected from the early appearance of myoglobin mRNA in embryonic skeletal muscle, both rat and mouse embryonic myoblasts accumulate myoglobin mRNA on fusion and differentiation in vitro.
我们发现，在人类和小鼠的肌生成过程中，肌红蛋白基因转录以及肌红蛋白的出现都非常早。与收缩蛋白基因不同的是，在随后的肌肉发育过程中，肌红蛋白mRNA和蛋白质水平会增加50至100倍。肌红蛋白和肌红蛋白mRNA在胎儿心脏中含量较高，在成人平滑肌中也能检测到低水平表达。在高度肌红蛋白化的海豹肌肉中，肌红蛋白mRNA的绝对水平非常高[占聚腺苷酸加尾RNA总数的2.8%]。海豹骨骼肌和各种人类肌肉类型中的肌红蛋白水平似乎由肌红蛋白mRNA池的大小决定。相比之下，小鼠骨骼肌中肌红蛋白水平较低，这显然与肌红蛋白mRNA水平较低无关。正如在胚胎骨骼肌中肌红蛋白mRNA早期出现所预期的那样，大鼠和小鼠胚胎成肌细胞在体外融合和分化时会积累肌红蛋白mRNA。
