State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
mSystems. 2023 Dec 21;8(6):e0047123. doi: 10.1128/msystems.00471-23. Epub 2023 Nov 15.
African swine fever (ASF), caused by African swine fever virus (ASFV), has become a major crisis for the pork industry in recent years. The mechanism for ASFV pathology and the clinical symptoms difference of ASF between domestic pigs and reservoir hosts remain to be elucidated. We deciphered the comprehensive protein-protein interaction (PPI) network between ASFV and host immune pathways. The intensive PPI network contained both ASFV-host immune pathway PPI and ASFV-ASFV PPI information, providing a comprehensive ASFV-host interaction landscape. Furthermore, the ASFV-host PPI difference between domestic pigs and warthogs was explored, which will be instructive for exploring essential candidates involved in ASFV pathology. Moreover, we screened the inhibitory effect of ASFV proteins in the PPI with cGAS-STING pathway on IFN-I and NF-κB, further providing possible functions of ASFV-host PPI network in innate immune regulation.
非洲猪瘟（ASF）由非洲猪瘟病毒（ASFV）引起，近年来已成为养猪业的重大危机。ASFV 的发病机制和 ASF 在国内猪和宿主之间的临床症状差异仍有待阐明。我们破译了 ASFV 与宿主免疫途径之间的综合蛋白质-蛋白质相互作用（PPI）网络。密集的 PPI 网络包含 ASFV-宿主免疫途径 PPI 和 ASFV-ASFV PPI 信息，提供了全面的 ASFV-宿主相互作用景观。此外，还探索了国内猪和疣猪之间的 ASFV-宿主 PPI 差异，这将有助于探索参与 ASFV 发病机制的重要候选物。此外，我们筛选了 PPI 中 cGAS-STING 途径对 IFN-I 和 NF-κB 的 ASFV 蛋白的抑制作用，进一步提供了 ASFV-宿主 PPI 网络在先天免疫调节中的可能功能。
