Son Ye-Eun, Park Hee-Soo
School of Food Science and Biotechnology, Kyungpook National University, Daegu, Republic of Korea.
Department of Integrative Biology, Kyungpook National University, Daegu, Republic of Korea.
Mycobiology. 2020 Nov 4;49(2):95-104. doi: 10.1080/12298093.2020.1838115. eCollection 2021.
Species of the genus have a variety of effects on humans and have been considered industrial cell factories due to their prominent ability for manufacturing several products such as heterologous proteins, secondary metabolites, and organic acids. Scientists are trying to improve fungal strains and re-design metabolic processes through advanced genetic manipulation techniques and gene delivery systems to enhance their industrial efficiency and utility. In this review, we describe the current status of the genetic manipulation techniques and transformation methods for species of the genus . The host strains, selective markers, and experimental materials required for the genetic manipulation and fungal transformation are described in detail. Furthermore, the advantages and disadvantages of these techniques are described.
该属的物种对人类有多种影响，并且由于它们在制造几种产品（如异源蛋白质、次生代谢产物和有机酸）方面具有突出能力，已被视为工业细胞工厂。科学家们正试图通过先进的基因操作技术和基因传递系统来改良真菌菌株并重新设计代谢过程，以提高它们的工业效率和实用性。在这篇综述中，我们描述了该属物种的基因操作技术和转化方法的现状。详细介绍了基因操作和真菌转化所需的宿主菌株、选择标记和实验材料。此外，还描述了这些技术的优缺点。
