Zhou Ying, Gao Xiujun, Shi Cuijuan, Li Mengying, Jia Wenwen, Shao Zongze, Yan Peisheng
School of Marine Science and Technology, Harbin Institute of Technology, Weihai, China.
China Key Laboratory of Marine Genetic Resources, The Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, China.
Mycobiology. 2021 Feb 11;49(2):151-160. doi: 10.1080/12298093.2020.1871175. eCollection 2021.
Despite recent studies, relatively few are known about the diversity of fungal communities in the deep Atlantic Ocean. In this study, we investigated the diversity of fungal communities in 15 different deep-sea sediments from the South Atlantic Ocean with a culture-dependent approach followed by phylogenetic analysis of ITS sequences. A total of 29 fungal strains were isolated from the 15 deep-sea sediments. These strains belong to four fungal genera, including , , , and . , accounting for 44.8% of the total fungal isolates, was a dominant genus. The antiaflatoxigenic activity of these deep-sea fungal isolates was studied. Surprisingly, most of the strains showed moderate to strong antiaflatoxigenic activity. Four isolates, belonging to species of , , and , could completely inhibit not only the mycelial growth of mutant strain NFRI-95, but also the aflatoxin production. To our knowledge, this is the first report to investigate the antiaflatoxigenic activity of culturable deep-sea fungi. Our results provide new insights into the community composition of fungi in the deep South Atlantic Ocean. The high proportion of strains that displayed antiaflatoxigenic activity demonstrates that deep-sea fungi from the Atlantic Ocean are valuable resources for mining bioactive compounds.
尽管近期有相关研究，但对于大西洋深处真菌群落的多样性，人们了解得还相对较少。在本研究中，我们采用依赖培养的方法，随后对ITS序列进行系统发育分析，调查了南大西洋15种不同深海沉积物中真菌群落的多样性。从这15种深海沉积物中总共分离出29株真菌菌株。这些菌株属于四个真菌属，包括 、 、 和 。 是优势属，占真菌分离株总数的44.8%。我们研究了这些深海真菌分离株的抗黄曲霉毒素活性。令人惊讶的是，大多数菌株表现出中度到强烈的抗黄曲霉毒素活性。四株属于 、 和 物种的分离株不仅能完全抑制 突变株NFRI-95的菌丝生长，还能抑制黄曲霉毒素的产生。据我们所知，这是第一份研究可培养深海真菌抗黄曲霉毒素活性的报告。我们的结果为南大西洋深处真菌的群落组成提供了新的见解。显示出抗黄曲霉毒素活性的菌株比例很高，这表明来自大西洋的深海真菌是挖掘生物活性化合物的宝贵资源。
