Kai Wang, Peisheng Yan, Rui Ma, Wenwen Jia, Zongze Shao
a School of Marine Science and Technology, Harbin Institute of Technology , Weihai , People's Republic of China.
b School of Municipal and Environmental Engineering, Harbin Institute of Technology , Harbin , People's Republic of China.
Bioengineered. 2017 Sep 3;8(5):572-584. doi: 10.1080/21655979.2017.1284711. Epub 2017 Jan 31.
The variability of culturable bacterial diversity and distribution was studied by phylogenetic analysis of 16S rRNA sequences. Seventeen water samples were examined and were collected, from different depths in the range of 5 m to 2700 m at 3 sampling sites (CTD06, CTD10 and CTD11) in the South Atlantic Ocean. Phylogenetic analysis of 16S rRNA gene sequences revealed a significant diversity of culturable bacteria. A total of 247 strains clustered into 8 classes: γ-Proteobacteria, α-Proteobacteria, Actinobacteria, Actinomycetales, Bacilli, Flavobacteria, Opitutae and Sphingobacteria. The 17 water samples were dominated by populations of strains belonging to the genus Erythrobacter (16.60%). Of the 247 strains, 10 were potential new species and might form a minor population in the deep sea. To our knowledge, this is the first report to analyze the diversity of culturable bacteria in the South Atlantic Ocean from different depths across the water column.
通过对16S rRNA序列进行系统发育分析，研究了可培养细菌多样性和分布的变异性。在南大西洋的3个采样点（CTD06、CTD10和CTD11）采集了17份水样，这些水样来自5米至2700米不同深度。16S rRNA基因序列的系统发育分析显示可培养细菌具有显著的多样性。总共247株菌株聚类为8个类群：γ-变形菌纲、α-变形菌纲、放线菌门、放线菌目、芽孢杆菌纲、黄杆菌纲、疣微菌门和鞘脂杆菌纲。17份水样中以属于红杆菌属的菌株群体为主（16.60%）。在这247株菌株中，有10株可能是新物种，在深海中可能构成一个较小的群体。据我们所知，这是第一份分析南大西洋不同深度水柱中可培养细菌多样性的报告。
