Faculty of Science, Department of Chemistry, Ankara University, 06100, Ankara, Turkey.
Graduate School of Natural and Applied Sciences, Ankara University, Ankara, Turkey.
Mikrochim Acta. 2023 Nov 17;190(12):471. doi: 10.1007/s00604-023-06054-w.
This study aims to develop a MIP-Apt-based electrochemical biosensor for the sensitive and selective determination of Lysozyme (Lyz), a food allergen. For the development of the sensor, in the first stage, modifications were made to the screen-printed electrode (SPE) surface with graphene oxide (GO) and gold nanoparticles (AuNPs) to increase conductivity and surface area. The advantages of using aptamer (Apt) and molecularly imprinted polymer (MIP) technology were combined in a single biointerface in the prepared sensing tool. Surface characterization of the biosensor was performed using scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray photoelectron spectrometry (XPS), contact angle measurements, cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS). A wide linear range from 0.001 to 100 pM was obtained under optimized conditions for the determination of Lyz detection using the proposed MIP-Apt sensing strategy. The limit of detection (LOD) and limit of quantification (LOQ) for Lyz were 3.67 fM and 12 fM, respectively. This biosensor displays high selectivity, repeatability, reproducibility, and long storage stability towards Lyz detection. The results show that a sensitive and selective sensor fabrication is achieved compared with existing methods.
本研究旨在开发一种基于分子印迹适体的电化学生物传感器，用于灵敏和选择性地测定食品过敏原溶菌酶（Lyz）。为了研制该传感器，在第一阶段，通过在丝网印刷电极（SPE）表面修饰氧化石墨烯（GO）和金纳米粒子（AuNPs）来提高导电性和比表面积。在制备的传感工具中，将适体（Apt）和分子印迹聚合物（MIP）技术的优点结合在单个生物界面中。通过扫描电子显微镜（SEM）、原子力显微镜（AFM）、X 射线光电子能谱（XPS）、接触角测量、循环伏安法（CV）、差分脉冲伏安法（DPV）和电化学阻抗谱（EIS）对生物传感器进行了表面特性表征。在优化条件下，使用所提出的 MIP-Apt 传感策略进行 Lyz 检测，可获得从 0.001 到 100 pM 的宽线性范围。Lyz 的检测限（LOD）和定量限（LOQ）分别为 3.67 fM 和 12 fM。该生物传感器对 Lyz 的检测表现出高选择性、重复性、重现性和长期存储稳定性。结果表明，与现有方法相比，实现了一种灵敏和选择性的传感器制备。
