牛丘疹性口炎病毒作为牛的疫苗载体。
Bovine papular stomatitis virus as a vaccine vector for cattle.
机构信息
School of Veterinary Medicine & Biomedical Sciences, University of Nebraska, Lincoln, Nebraska, USA.
Department of Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana, Illinois, USA.
出版信息
J Gen Virol. 2023 Nov;104(11). doi: 10.1099/jgv.0.001914.
Virus vectored vaccines are not available commercially for cattle even though compelling potential applications exist. Bovine papular stomatitis virus (BPSV), a highly prevalent parapoxvirus, causes self-limited oral lesions in cattle. Ability of virus to accommodate large amounts of foreign DNA, induce low level of antiviral immunity, and circulate and likely persist in cattle populations, make BPSV an attractive candidate viral vector. Here, recombinant BPSV were constructed expressing either Bovine herpesvirus 1 (BoHV-1) glycoprotein gD (BPSV), or gD and gB (BPSV). Immunization of BPSV serologically-positive calves with BPSV or BPSV induced BoHV-1 neutralization antibodies and provided protection for three of four animals following a high dose BoHV-1 challenge at day 70 pi. Results indicate BPSV suitability as a candidate virus vector for cattle vaccines.
病毒载体疫苗目前尚未在牛中商业化应用，尽管具有很大的潜在应用价值。牛丘疹性口炎病毒（BPSV）是一种高流行的副痘病毒，可引起牛的自限性口腔病变。该病毒能够容纳大量的外源 DNA，诱导低水平的抗病毒免疫，在牛群中循环并可能持续存在，使其成为有吸引力的候选病毒载体。在这里，构建了表达牛疱疹病毒 1（BoHV-1）糖蛋白 gD（BPSV）或 gD 和 gB（BPSV）的重组 BPSV。用 BPSV 或 BPSV 对 BPSV 血清学阳性小牛进行免疫接种，可诱导 BoHV-1 中和抗体，并在 70 天接种后高剂量 BoHV-1 攻毒时为 4 只动物中的 3 只提供保护。结果表明 BPSV 适合作为牛疫苗的候选病毒载体。
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