Department of Materials Science and Engineering, College of Chemistry and Materials Science, Jinan University, Guangzhou 511443, China.
Guangdong Provincial Key Laboratory of Functional Supramolecular Coordination Materials and Applications, College of Chemistry and Materials Science, Jinan University, Guangzhou 510632, China.
J Colloid Interface Sci. 2024 Feb;655:736-747. doi: 10.1016/j.jcis.2023.11.067. Epub 2023 Nov 11.
Copper-based Fenton-like agents have the ability to convert weakly oxidizing HO into highly oxidizing hydroxyl radicals (·OH) at tumor sites during chemodynamic therapy (CDT). In this study, the interfacial attraction properties between the negatively charged OCP in sodium phosphathynolate (NaOCP) and the positively charged environment inside the lumen of halloysite nanotubes (HNTs) were utilized to synthesize CuP nanoparticles in situ within the HNTs. The study investigated the chemical composition, morphology, and structure of Cu P@HNTs. The results indicated uniform distribution of CuP particles measuring 3-5 nm within HNTs' lumen. Experiments conducted internally and externally to cells confirmed the catalytic capability of CuP@HNTs to oxidize HO to ·OH. Furthermore, CP@H-CM was synthesized by enclosing CuP@HNTs in a cancer cell membrane, which selectively targets cancer cells. The experiments revealed the cytotoxicity of CP@H-CM on 4T1 cells. Additionally, the antitumor efficacy of CP@H-CM was evaluated in vivo through tumor recurrence experiments in mice. Moreover, the efficacy of CP@H-CM in repressing tumor growth was enhanced by incorporating infrared laser, indicating a synergistic photodynamic treatment for breast cancer. This study presents an efficacious and viable therapeutic approach to inhibit postoperative tumor reappearance. The implications of this approach are promising, particularly in the domain of tumor treatment and metastasis.
铜基芬顿样试剂在化学动力学治疗（CDT）中具有在肿瘤部位将弱氧化的 HO 转化为强氧化的羟基自由基（·OH）的能力。在本研究中，利用负电荷的磷氧四面体（NaOCP）中的界面吸引力特性和 halloysite 纳米管（HNTs）管腔内的正电荷环境，在 HNTs 内原位合成了 CuP 纳米颗粒。研究了 CuP@HNTs 的化学组成、形态和结构。结果表明，CuP 颗粒均匀分布在 HNTs 管腔内，粒径为 3-5nm。细胞内外实验证实了 CuP@HNTs 将 HO 氧化为·OH 的催化能力。此外，通过将 CuP@HNTs 封装在癌细胞膜内合成了 CP@H-CM，CP@H-CM 能够选择性地靶向癌细胞。实验揭示了 CP@H-CM 对 4T1 细胞的细胞毒性。此外，通过在小鼠中进行肿瘤复发实验评估了 CP@H-CM 的体内抗肿瘤功效。此外，通过结合红外激光增强 CP@H-CM 的抑制肿瘤生长功效，表明其对乳腺癌具有协同光动力治疗作用。本研究提出了一种有效且可行的治疗方法，可抑制术后肿瘤复发。这种方法具有广阔的应用前景，特别是在肿瘤治疗和转移领域。
