Gong Ting, Wu Dongdong, Feng Yongzhi, Liu Xing, Gao Qi, Zheng Xiaoyu, Song Zebu, Wang Heng, Zhang Guihong, Gong Lang
Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China; Zhaoqing Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, China.
Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture and Rural Affairs, Guangzhou, 510000, China.
Virology. 2024 Jan;589:109923. doi: 10.1016/j.virol.2023.109923. Epub 2023 Nov 7.
Porcine epidemic diarrhea (PED) is an acute, severe, highly contagious disease. Porcine epidemic diarrhea virus (PEDV) strains are prone to mutation, and the immune response induced by traditional vaccines may not be strong enough to be effective against the virus. Therefore, there is an urgent need to develop novel anti-PEDV drugs. This study aimed to explore the therapeutic effects of quercetin in PEDV infections in vitro (Vero cells) and in vivo (suckling piglets). Using transmission electron microscopy and laser confocal microscopy, we found that PEDV infection promotes the accumulation of lipid droplets (LDs). In vitro, studies showed that quercetin inhibits LD accumulation by down-regulating NF-κB signaling and IL-1β, IL-8, and IL-6 levels, thereby inhibiting viral replication. In vivo, studies in pigs demonstrated that quercetin can effectively relieve the clinical symptoms and intestinal injury caused by PEDV. Collectively, our findings suggest that quercetin inhibits PEDV replication both in vivo and in vitro, which provides a new direction for the development of PED antiviral drugs.
猪流行性腹泻（PED）是一种急性、严重、高度传染性疾病。猪流行性腹泻病毒（PEDV）毒株易于突变，传统疫苗诱导的免疫反应可能不够强大，无法有效对抗该病毒。因此，迫切需要开发新型抗PEDV药物。本研究旨在探讨槲皮素在体外（Vero细胞）和体内（哺乳仔猪）对PEDV感染的治疗效果。通过透射电子显微镜和激光共聚焦显微镜，我们发现PEDV感染促进脂滴（LDs）的积累。在体外，研究表明槲皮素通过下调NF-κB信号通路以及IL-1β、IL-8和IL-6水平来抑制脂滴积累，从而抑制病毒复制。在体内，对猪的研究表明槲皮素可以有效缓解PEDV引起的临床症状和肠道损伤。总体而言，我们的研究结果表明槲皮素在体内和体外均能抑制PEDV复制，这为开发PED抗病毒药物提供了新方向。
