State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, China.
Int J Mol Sci. 2020 Oct 30;21(21):8095. doi: 10.3390/ijms21218095.
For the last decade, porcine epidemic diarrhea virus (PEDV) variant strains have caused severe damage to the global pig industry. Until now, no effective antivirals have been developed for the therapeutic treatment of PEDV infection. In the present study, we found that quercetin significantly suppressed PEDV infection at noncytotoxic concentrations. A molecular docking study indicated that quercetin might bind the active site and binding pocket of PEDV 3C-like protease (3CL). Surface plasmon resonance (SPR) analysis revealed that quercetin exhibited a binding affinity to PEDV 3CL. Based on the results of the fluorescence resonance energy transfer (FRET) assay, quercetin was proven to exert an inhibitory effect on PEDV 3CL. Since coronavirus 3CL is an important drug target and participates in the viral replication process, quercetin should be developed as a novel drug in the control of PEDV infection.
在过去的十年中，猪流行性腹泻病毒（PEDV）变异株对全球养猪业造成了严重的破坏。到目前为止，还没有开发出有效的抗病毒药物来治疗 PEDV 感染。在本研究中，我们发现槲皮素在非细胞毒性浓度下能显著抑制 PEDV 感染。分子对接研究表明，槲皮素可能与 PEDV 3C 样蛋白酶（3CL）的活性位点和结合口袋结合。表面等离子体共振（SPR）分析表明，槲皮素对 PEDV 3CL 具有结合亲和力。基于荧光共振能量转移（FRET）测定的结果，证明槲皮素对 PEDV 3CL 发挥抑制作用。由于冠状病毒 3CL 是一个重要的药物靶点，参与病毒复制过程，槲皮素应该被开发成一种控制 PEDV 感染的新型药物。
