Ewies Samar S, Tamam Sabry M, Abdel-Moneim Ahmed S, Rouby Sherin R
Department of Virology, Faculty of Veterinary Medicine, Beni-Suef University, Beni Suef, 62511, Egypt.
Department of Microbiology, College of Medicine, Taif University, Al-Taif, Saudi Arabia.
Virology. 2024 Jan;589:109924. doi: 10.1016/j.virol.2023.109924. Epub 2023 Nov 7.
Contagious Ecthyma (CE) is a highly contagious viral disease of sheep and goats with worldwide distribution. The present study aimed to provide a clinical description of contagious ecthyma in four sheep flocks and screen the possible genetic variation in the B2L gene of the detected isolates. Oral lesions were collected and inoculated into chorioallantoic membrane (CAM) of 11 days embryonated chicken eggs. Polymerase chain reaction and direct sequencing of the B2L gene was conducted. Infected sheep exhibited anorexia with a development of nodular lesions evolving in proliferative thick scabs around oral commissures. The inoculated CAM showed small-sized white pock lesions accompanied with thickening of CAM. The partial length of B2L gene (592 bp) was successfully amplified in samples collected from four flocks. The isolated strains belong to genotype I/I and I/II. Sequence and evolutionary analysis illustrate that B2L gene (ORF011) are highly conserved among Orf viruses isolated from different countries.
传染性脓疱性皮炎（CE）是一种在全球范围内分布的绵羊和山羊高度传染性病毒性疾病。本研究旨在对四个绵羊群中的传染性脓疱性皮炎进行临床描述，并筛选检测到的分离株B2L基因中可能存在的基因变异。采集口腔病变样本并接种到11日龄鸡胚的绒毛尿囊膜（CAM）上。进行了B2L基因的聚合酶链反应和直接测序。受感染的绵羊表现出厌食，在口角周围出现结节性病变，并发展为增殖性厚痂。接种的CAM显示出小尺寸的白色痘疱病变，同时伴有CAM增厚。从四个羊群采集的样本中成功扩增出B2L基因的部分长度（592 bp）。分离出的菌株属于基因型I/I和I/II。序列和进化分析表明，B2L基因（ORF011）在从不同国家分离的羊口疮病毒中高度保守。
