Markham A F, Porter R A, Gait M J, Sheppard R C, Kerr I M
Nucleic Acids Res. 1979 Jun 11;6(7):2569-82. doi: 10.1093/nar/6.7.2569.
Specific synthesis of some oligoadenylates including A2'p5'A2'p5'Ap(2'), the 2'-phosphorylated oligoribonucleotide core of the recently discovered protein synthesis inhibitor pppA2'p5'A2'p5'A is described using a novel solid-phase method. The CD spectra of A2'p5'Ap(2'), A2'p5'A2'p5'Ap(2') and A2'p5'A2'p5'A (derived by treatment of the phosphorylated synthetic trimer with E. coli alkaline phosphatase) are presented. Comparison of the latter spectrum with that of A2'p5'A2'p5'A obtained similarly from a biologically derived sample of pppA2'p5'A2'p5'A provides further evidence that this molecule is in fact the first naturally-occurring 2'-5'-linked oligoribonucleotide.
本文描述了使用一种新型固相方法特异性合成一些寡聚腺苷酸,包括A2'p5'A2'p5'Ap(2'),即最近发现的蛋白质合成抑制剂pppA2'p5'A2'p5'A的2'-磷酸化寡核糖核苷酸核心。给出了A2'p5'Ap(2')、A2'p5'A2'p5'Ap(2')和A2'p5'A2'p5'A(通过用大肠杆菌碱性磷酸酶处理磷酸化合成三聚体得到)的圆二色光谱。将后一种光谱与从生物来源的pppA2'p5'A2'p5'A样品中类似获得的A2'p5'A2'p5'A光谱进行比较,进一步证明该分子实际上是第一个天然存在的2'-5'-连接的寡核糖核苷酸。