对转运核糖核酸中发现的2-硫代嘧啶核苷酸构象特征的核磁共振氢谱研究。
1H NMR studies on the conformational characteristics of 2-thiopyrimidine nucleotides found in transfer RNAs.
作者信息
Yokoyama S, Yamaizumi Z, Nishimura S, Miyazawa T
出版信息
Nucleic Acids Res. 1979 Jun 11;6(7):2611-26. doi: 10.1093/nar/6.7.2611.
Abstract
The molecular conformations of naturally occurring 2-thiopyrimidine nucleosides (5-methylaminomethyl-2-thiouridine, 5-methoxycarbonylmethyl-2-thiouridine and 2-thiocytidine) and 5'-mononucleotides (5-methylaminomethyl-2-thiouridine 5'-monophosphate and 2-thiocytidine 5'-monophosphate) in 2H2O solution were elucidated by analyses of the proton NMR spin-coupling constant, nuclear Overhauser effect, and lanthanide-induced shifts and relaxation enhancements. As monomers, these nucleotides are almost exclusively in the 3E-gg-anti form, even in the absence of ordinary stabilizing factors of this form; i. e., base-stacking and base-pairing interactions with other nucleotide units. This inherent conformational rigidity of the 2-thiopyrimidine units probably contributes to stability of the conformation of tRNA.
摘要
通过对质子核磁共振自旋耦合常数、核Overhauser效应、镧系元素诱导位移和弛豫增强的分析，阐明了天然存在的2-硫代嘧啶核苷（5-甲基氨基甲基-2-硫代尿苷、5-甲氧基羰基甲基-2-硫代尿苷和2-硫代胞苷）以及5'-单核苷酸（5-甲基氨基甲基-2-硫代尿苷5'-单磷酸和2-硫代胞苷5'-单磷酸）在2H2O溶液中的分子构象。作为单体，这些核苷酸几乎完全处于3E-gg-反式构象，即使在缺乏这种构象的常见稳定因素（即与其他核苷酸单元的碱基堆积和碱基配对相互作用）的情况下也是如此。2-硫代嘧啶单元这种固有的构象刚性可能有助于tRNA构象的稳定性。
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本文引用的文献
1
A new polynucleotide complex poly(s 2 C)-poly(I).一种新的多核苷酸复合物聚（s2C）-聚（I）。
Eur J Biochem. 1972 May;27(1):109-15. doi: 10.1111/j.1432-1033.1972.tb01816.x.
2
Conformation of the exocyclic 5'-CH 2 OH in nucleosides and nucleotides in aqueous solution from specific assignments of the H 5' and H 5'' signals in the NMR spectra.通过核磁共振谱中H5'和H5''信号的特定归属确定水溶液中核苷和核苷酸外环5'-CH2OH的构象。
Biochem Biophys Res Commun. 1972 Aug 7;48(3):636-42. doi: 10.1016/0006-291x(72)90395-6.
3
The synthesis of the alternating copolymer poly(r(A-s4U)) by RNA polymerase of Escherichia coli.
Eur J Biochem. 1971 Apr;19(3):379-85. doi: 10.1111/j.1432-1033.1971.tb01327.x.
4
The enzymatic synthesis of poly 4-thiouridylic acid by polynucleotide phosphorylase from Escherichia coli.大肠杆菌多核苷酸磷酸化酶催化合成聚4-硫代尿苷酸。
Biochim Biophys Acta. 1970 Apr 15;204(2):371-80. doi: 10.1016/0005-2787(70)90156-5.
5
Molecular structure of poly-2-thiouridylic acid, a double helix with non-equivalent polynucleotide chains.聚-2-硫代尿苷酸的分子结构，一种具有不等同多核苷酸链的双螺旋结构。
J Mol Biol. 1974 May 15;85(2):213-9. doi: 10.1016/0022-2836(74)90361-1.
6
Structures of synthetic polynucleotides in the A-RNA and A'-RNA conformations: x-ray diffraction analyses of the molecular conformations of polyadenylic acid--polyuridylic acid and polyinosinic acid--polycytidylic acid.A-RNA和A'-RNA构象的合成多核苷酸结构：聚腺苷酸-聚尿苷酸和聚肌苷酸-聚胞苷酸分子构象的X射线衍射分析
J Mol Biol. 1973 Dec 5;81(2):107-22. doi: 10.1016/0022-2836(73)90183-6.
7
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J Am Chem Soc. 1973 Apr 4;95(7):2333-44. doi: 10.1021/ja00788a038.
8
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9
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Eur J Biochem. 1973 Mar 15;33(3):535-44. doi: 10.1111/j.1432-1033.1973.tb02713.x.
10
Nuclear magnetic resonance studies of the solution conformation of nucleoside diphosphohexoses and their components.核苷二磷酸己糖及其组分溶液构象的核磁共振研究。
Biochemistry. 1976 Feb 10;15(3):697-704. doi: 10.1021/bi00648a038.
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