Faculty of Engineering, Yokohama National University, 79-5 Tokiwadai, Hodogaya-ku, Yokohama 240-8501, Japan.
Graduate School of Engineering Science, Yokohama National University, 79-5 Tokiwadai, Hodogaya-ku, Yokohama 240-8501, Japan.
ACS Biomater Sci Eng. 2024 Feb 12;10(2):762-772. doi: 10.1021/acsbiomaterials.3c01046. Epub 2023 Nov 20.
To construct a complex three-dimensional (3D) structure mimicking bone microstructure, hydrogel models of polymerized gelatin methacrylate (pGelMA) were fabricated by using stereolithography and modified with hydroxyapatite (HAp) via an alternate soaking process (ASP) using a solution of calcium and phosphate ions. Fabricated pGelMA line models whose widths were designed as 100, 300, and 600 μm were modified with HAp by ASP by changing the immersion time and number of cycles. After ASP, all of the line models with widths of 100, 300, and 600 μm were successfully modified with HAp, and large amounts of HAp were covered with the fabricated models by increasing both the immersion time and the number of cycles in ASP. HAp was observed near the surface of the line model with a width of 600 μm after ASP at an immersion time of 10 s, while the entire model was modified with HAp using ASPs for longer immersion times. The adhesion and spread of mesenchymal stem cells (MSCs) on the pGelMA-HAp discs depended on the ASP conditions. Moreover, the HAp modification of 3D pyramid models without alteration of the microstructure was also conducted. This two-step fabrication method of first fabricating frameworks of hydrogel models by stereolithography and subsequently modifying the fabricated models with HAp will lead to the development of 3D cell culture systems to support bone grafts or to create biological niches, such as artificial bone marrow.
为了构建模仿骨微观结构的复杂三维（3D）结构，使用立体光刻技术制造了聚合明胶甲基丙烯酸酯（pGelMA）的水凝胶模型，并通过使用钙和磷酸盐离子溶液的交替浸泡工艺（ASP）对其进行羟基磷灰石（HAp）改性。设计宽度为 100、300 和 600 μm 的 pGelMA 线模型通过改变浸泡时间和循环次数通过 ASP 进行 HAp 改性。ASP 后，所有宽度为 100、300 和 600 μm 的线模型均成功地用 HAp 改性，并且通过增加 ASP 中的浸泡时间和循环次数，大量的 HAp 覆盖了所制造的模型。在宽度为 600 μm 的线模型上进行 10 s 的 ASP 后，在 ASP 后可以在线模型表面附近观察到 HAp，而使用更长的浸泡时间的 ASP 则可以对整个模型进行 HAp 改性。间充质干细胞（MSCs）在 pGelMA-HAp 盘上的粘附和扩散取决于 ASP 条件。此外，还对微观结构不变的 3D 金字塔模型进行了 HAp 改性。这种两步制造方法首先通过立体光刻技术制造水凝胶模型的框架，然后用 HAp 对所制造的模型进行改性，将导致开发 3D 细胞培养系统，以支持骨移植物或创建生物龛，例如人工骨髓。
