Choi Ji-Bong, Kim Yu-Kyoung, Byeon Seon-Mi, Park Jung-Eun, Bae Tae-Sung, Jang Yong-Seok, Lee Min-Ho
Department of Dental Biomaterials, Institute of Biodegradable Materials, School of Dentistry, Jeonbuk National University, Jeonju-si 54896, Jeollabuk-do, Korea.
Dental Clinic of Ebarun, Suncheon-si 57999, Jeollanam-do, Korea.
Nanomaterials (Basel). 2021 Mar 2;11(3):617. doi: 10.3390/nano11030617.
In the field of bone tissue, maintaining adequate mechanical strength and tissue volume is an important part. Recently, biphasic calcium phosphate (BCP) was fabricated to solve the shortcomings of hydroxyapatite (HA) and beta-tricalcium phosphate (β-TCP), and it is widely studied in the field of bone-tissue engineering. In this study, a composite hydrogel was fabricated by applying BCP to gelatin methacrylate (GelMA). It was tested by using a mechanical tester, to characterize the mechanical properties of the prepared composite hydrogel. The fabricated BCP was analyzed through FTIR and XRD. As a result, a different characteristic pattern from hydroxyapatite (HA) and beta-tricalcium phosphate (β-TCP) was observed, and it was confirmed that it was successfully bound to the hydrogel. Then, the proliferation and differentiation of preosteoblasts were checked to evaluate cell viability. The analysis results showed high cell viability and relatively high bone differentiation ability in the composite hydrogel to which BCP was applied. These features have been shown to be beneficial for bone regeneration by maintaining the volume and shape of the hydrogel. In addition, hydrogels can be advantageous for clinical use, as they can shape the structure of the material for custom applications.
在骨组织领域，维持足够的机械强度和组织体积是重要的一环。最近，双相磷酸钙（BCP）被制备出来以解决羟基磷灰石（HA）和β-磷酸三钙（β-TCP）的缺点，并且它在骨组织工程领域得到了广泛研究。在本研究中，通过将BCP应用于甲基丙烯酸明胶（GelMA）制备了一种复合水凝胶。使用力学测试仪对其进行测试，以表征所制备复合水凝胶的力学性能。通过傅里叶变换红外光谱（FTIR）和X射线衍射（XRD）对制备的BCP进行分析。结果，观察到了与羟基磷灰石（HA）和β-磷酸三钙（β-TCP）不同的特征图谱，并证实其成功地与水凝胶结合。然后，检查前成骨细胞的增殖和分化以评估细胞活力。分析结果表明，在应用了BCP的复合水凝胶中细胞活力高且骨分化能力相对较高。这些特性已被证明通过维持水凝胶的体积和形状对骨再生有益。此外，水凝胶在临床应用中可能具有优势，因为它们可以根据定制应用塑造材料的结构。
