Graduation Program of Pharmaceutical Sciences, State University of Ponta Grossa, Ponta Grossa, PR, Brazil.
Department of Medicine, State University of Ponta Grossa, Ponta Grossa, PR, Brazil.
Chem Biodivers. 2023 Dec;20(12):e202301243. doi: 10.1002/cbdv.202301243. Epub 2023 Nov 29.
Leonurus japonicus Houtt. is a medicinal plant popular in Brazil as "rubim", used in local folk medicine for several applications as an anti-inflammatory, antioxidant, analgesic, and antimicrobial phytomedicine. The traditional use for wound healing is related; however, few studies have evaluated the wound healing activity. Thus, this study aimed to analyse the popular indication of the hydroalcoholic and aqueous extracts of L. japonicus aerial parts in a rat wound healing model. The initial chemical characterization was performed using flavonoid quantification and complemented with mass spectroscopy/chemometrics analysis. The wound's lesion contraction and tissue regeneration (histological study stained with hematoxylin-eosin and picrosirius) were determined. Hydroalcoholic and aqueous extracts presented high flavonoid content, and mass spectrometry analysis of the extracts demonstrated the presence of compounds with a mass between 100-650, reinforcing the presence of polyphenolic constituents. The extracts of L. japonicus improve various wound healing phases, like inflammatory modulation, wound contraction, and collagen synthesis, resulting in faster healing in rats. These effects could be related to the extracts' polyphenolic compounds.
益母草是一种在巴西广受欢迎的药用植物，被称为“rubim”，用于当地民间医学的多种应用，如抗炎、抗氧化、镇痛和抗菌植物药。传统上用于伤口愈合的应用与之相关；然而，很少有研究评估其伤口愈合活性。因此，本研究旨在分析益母草地上部分的水醇提物和水提物在大鼠伤口愈合模型中的流行适应症。初始化学表征使用黄酮类定量法进行，并辅以质谱/化学计量学分析。通过伤口病变收缩和组织再生（苏木精-伊红和苦味酸染色的组织学研究）来确定。水醇提物和水提物均具有较高的黄酮类化合物含量，提取物的质谱分析表明存在质量在 100-650 之间的化合物，这加强了多酚类成分的存在。益母草提取物可改善炎症调节、伤口收缩和胶原蛋白合成等各种伤口愈合阶段，从而使大鼠更快愈合。这些作用可能与提取物中的多酚类化合物有关。
