Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
J Ethnopharmacol. 2024 Feb 10;320:117424. doi: 10.1016/j.jep.2023.117424. Epub 2023 Nov 19.
Maxing Shigan Decoction (MXSG) is a traditional Chinese Medicine effectively used in respiratory infections and bacterial pneumonia. However, the mechanism of MXSG treating acute Pseudomonas aeruginosa (P. aeruginosa) pneumonia is still unclear.
This study aimed to investigate the therapeutic effects of MXSG on acute P. aeruginosa pneumonia and explore its potential mechanisms.
HPLC-MS analysis was performed to analyze the chemical composition. Antibacterial effects in vitro were evaluated by minimum inhibitory concentration (MIC). Forty-five male BALB/c mice were divided into control group, model group, levofloxacin group, MXSG-L (7.7 g/kg/d), and MXSG-H group (15.4 g/kg/d). Mice were intranasal instillation with P. aeruginosa to induce acute P. aeruginosa pneumonia model. Levofloxacin and MXSG were administered by oral gavage once a day. After 3 days of treatment, the lung index measurement, micro-CT, arterial blood gas analysis, bacteria load determination, and HE staining were performed. Network pharmacological analysis and transcriptome sequencing were employed to predict the potential mechanisms of MXSG on bacterial pneumonia. The expressions of relating genes were detected by immunofluorescence, Western blot, and RT-PCR.
In vitro, MIC of P. aeruginosa is greater than 500 mg/mL. In the treatment of acute P. aeruginosa pneumonia model, MXSG significantly improved body weight loss, lung index, and pulmonary lesions. MXSG treatment also reduced the bacterial load and ameliorated oxygen saturation significantly. Transcriptomes, immunofluorescence, Western blot, and RT-PCR analysis showed MXSG treating acute P. aeruginosa pneumonia through the IL-17 signaling pathway and HIF-1α/IL-6/STAT3 signaling pathway.
We demonstrated the efficacy and mechanism of MXSG in the treatment of acute P. aeruginosa pneumonia, which provides a scientific basis for its clinical application.
麻杏石甘汤（MXSG）是一种有效的中药，用于治疗呼吸道感染和细菌性肺炎。然而，MXSG 治疗急性铜绿假单胞菌（P. aeruginosa）肺炎的机制尚不清楚。
本研究旨在探讨 MXSG 治疗急性铜绿假单胞菌肺炎的疗效及其潜在机制。
采用 HPLC-MS 分析方法分析化学成分。采用最低抑菌浓度（MIC）评估体外抗菌作用。将 45 只雄性 BALB/c 小鼠分为对照组、模型组、左氧氟沙星组、MXSG-L（7.7 g/kg/d）组和 MXSG-H 组（15.4 g/kg/d）。通过鼻腔滴注铜绿假单胞菌诱导急性铜绿假单胞菌肺炎模型。每天口服灌胃给予左氧氟沙星和 MXSG。治疗 3 天后，进行肺指数测量、微 CT、动脉血气分析、细菌载量测定和 HE 染色。采用网络药理学分析和转录组测序预测 MXSG 治疗细菌性肺炎的潜在机制。通过免疫荧光、Western blot 和 RT-PCR 检测相关基因的表达。
体外研究显示，铜绿假单胞菌的 MIC 大于 500 mg/mL。在治疗急性铜绿假单胞菌肺炎模型中，MXSG 显著改善了体重减轻、肺指数和肺部病变。MXSG 治疗还显著降低了细菌负荷，提高了氧饱和度。转录组、免疫荧光、Western blot 和 RT-PCR 分析表明，MXSG 通过 IL-17 信号通路和 HIF-1α/IL-6/STAT3 信号通路治疗急性铜绿假单胞菌肺炎。
本研究证实了 MXSG 治疗急性铜绿假单胞菌肺炎的疗效及其作用机制，为其临床应用提供了科学依据。
