College of Chemistry and Chemical Engineering, College of Materials Science and Engineering, Institute of Biomedical Materials and Engineering, Qingdao University, Qingdao 266071, China.
College of Chemistry and Chemical Engineering, College of Materials Science and Engineering, Institute of Biomedical Materials and Engineering, Qingdao University, Qingdao 266071, China; State Key Laboratory of Bio-Fibers and Eco-Textiles, Qingdao University, Qingdao 266071, China; School of Materials Science and Engineering, Shandong University of Technology, Zibo 255000, China.
Carbohydr Polym. 2024 Jan 15;324:121562. doi: 10.1016/j.carbpol.2023.121562. Epub 2023 Nov 4.
Gene therapy, as a revolutionary treatment, has been gaining more and more attention. The key to gene therapy is the selection of suitable vectors for protection of exogenous nucleic acid molecules and enabling their specific release in target cells. While viral vectors have been widely used in researches, non-viral vectors are receiving more attention due to its advantages. Chitosan (CS) has been widely used as non-viral organic gene carrier because of its good biocompatibility and its ability to load large amounts of nucleic acids. This paper summarizes and evaluates the potential of chitosan and its derivatives as gene delivery vector materials, along with factors influencing transfection efficiency, performance evaluation, ways to optimize infectious efficiency, and the current main research development directions. Additionally, it provides an outlook on its future prospects.
基因治疗作为一种革命性的治疗方法，越来越受到关注。基因治疗的关键在于选择合适的载体来保护外源核酸分子，并使其在靶细胞中特异性释放。虽然病毒载体在研究中得到了广泛应用，但由于非病毒载体具有优势，因此越来越受到关注。壳聚糖 (CS) 由于具有良好的生物相容性和能够负载大量核酸的能力，已被广泛用作非病毒有机基因载体。本文综述和评价了壳聚糖及其衍生物作为基因传递载体材料的潜力，以及影响转染效率的因素、性能评价、优化感染效率的途径以及当前主要的研究发展方向。此外，还对其未来前景进行了展望。
