Section for Pharmaceutical Chemistry, Department of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, NO-0316 Oslo, Norway.
Department of Pharmacology, Institute of Clinical Medicine, University of Oslo, P.O. Box 1057, Blindern, NO-0316 Oslo, Norway.
Carbohydr Polym. 2024 Jan 15;324:121554. doi: 10.1016/j.carbpol.2023.121554. Epub 2023 Nov 3.
Daphne mezereum L., an important medicinal plant in Scandinavian folk medicine, was used to treat ailments such as diarrhea, swelling and stomach pain. A range of natural compounds have been isolated, but little attention has been given to the polysaccharides in this plant. Previous work in our group have shown that a polysaccharide enriched fraction from the bark of D. mezereum exhibited pro-inflammatory effects. To pursue this further, the aim of the present work was to isolate and characterize these polysaccharides. From the ethanol-precipitate of a water extract, one neutral (DMP-NF) and one acidic (DMP-AF) fraction was isolated by anion-exchange chromatography. GC, GC-MS and 1D- and 2D-NMR were used to characterize the polysaccharide structures. DMP-NF appeared to be a mixture of arabinan, arabinogalactan and hemicelluloses such as xyloglucan, mannan and xylan. DMP-AF contained a pectic polysaccharide mainly consisting of an unusually long homogalacturonan backbone. Enzymatic treatment by pectinase of DMP-AF yielded DMP-ED, which contained a rhamnogalacturonan-I backbone with arabinan, galactan and arabinogalactan side chains. Both DMP-NF and DMP-ED induced IFN-γ and TNF-α secretion in peripheral blood mononuclear cells (PBMCs), DMP-ED being the most potent fraction. DMP-AF was less active, which might be due to a less sterically available rhamnogalacturonan-I domain.
瑞香科植物 Daphne mezereum L. 是斯堪的纳维亚民间医学中的一种重要药用植物，用于治疗腹泻、肿胀和胃痛等疾病。已经分离出多种天然化合物，但对这种植物中的多糖却关注甚少。我们小组以前的工作表明，从 D. mezereum 树皮中分离出的富含多糖的部分具有促炎作用。为了进一步研究，本工作的目的是分离和表征这些多糖。从水提取物的乙醇沉淀中，通过阴离子交换层析分离得到一种中性（DMP-NF）和一种酸性（DMP-AF）级分。GC、GC-MS 以及 1D 和 2D-NMR 用于表征多糖结构。DMP-NF 似乎是阿拉伯聚糖、阿拉伯半乳聚糖和木聚糖、甘露聚糖和木聚糖等半纤维素的混合物。DMP-AF 含有一种果胶多糖，主要由异常长的同半乳糖醛酸聚糖主链组成。果胶酶处理 DMP-AF 得到 DMP-ED，它含有鼠李半乳糖醛酸聚糖 I 主链，带有阿拉伯聚糖、半乳糖聚糖和阿拉伯半乳聚糖侧链。DMP-NF 和 DMP-ED 均可诱导外周血单核细胞（PBMC）分泌 IFN-γ 和 TNF-α，DMP-ED 是最有效的部分。DMP-AF 的活性较低，这可能是由于其鼠李半乳糖醛酸聚糖 I 结构域的空间位阻较小。
