Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, PR China; Department of Systematic and Evolutionary Botany, University of Zurich, Zollikerstrasse 107, Zurich 8008, Switzerland.
Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, PR China.
Int J Biol Macromol. 2018 Jun;112:356-363. doi: 10.1016/j.ijbiomac.2018.01.207. Epub 2018 Feb 1.
Purification, characterization and biological activities of polysaccharides from Lycium barbarum pedicel were investigated in this study. Two polysaccharides, PLBP-I-I and PLBP-II-I, were obtained from water extracts by anion exchange chromatography and gel filtration. Structural elucidation based on IR, H NMR, and C NMR spectra indicated that these two fractions were typical pectic polysaccharides, with homogalacturonan and rhamnogalacturonan type I regions and arabinogalactan side chains, and some of the galacturonic acid units were methyl esterified. Both fractions exhibited potent complement fixating activity and pro-antioxidant defense capacity, and those two fractions showed different activities. The higher complement fixation activity was obtained in fraction PLBP-I-I, while the higher pro-antioxidant defense capacity was obtained in fraction PLBP-II-I, which may be due to the structural differences between those two fractions. Thus, the pedicel of L. barbarum could be used as a potential source for natural immunomodulator and antioxidant.
本研究旨在探讨宁夏枸杞梗中多糖的提取、分离纯化、结构鉴定及其生物活性。采用阴离子交换层析和凝胶过滤层析从水提物中分离得到两种多糖，命名为 PLBP-I-I 和 PLBP-II-I。基于红外光谱（IR）、核磁共振氢谱（1H NMR）和碳谱（13C NMR）分析结果，表明这两种多糖均属于典型的果胶多糖，具有同型半乳糖醛酸和鼠李半乳糖醛酸 I 型区域以及阿拉伯半乳聚糖侧链，部分半乳糖醛酸单元发生了甲酯化。两种多糖均具有较强的补体固定活性和抗氧化防御能力，且这两种多糖的活性存在差异。PLBP-I-I 具有较高的补体固定活性，而 PLBP-II-I 具有较高的抗氧化防御能力，这可能与两种多糖的结构差异有关。因此，宁夏枸杞梗可作为天然免疫调节剂和抗氧化剂的潜在来源。
