Instituto Latino-Americano de Ciências da Vida e da Natureza, Universidade Federal da Integração Latino-Americana, Paraná, 85870-901, Brazil.
Laboratory of Chemistry, Universidade Tecnológica Federal do Paraná - UTFPR, Câmpus Santa Helena, Santa Helena, Paraná, Brazil.
Exp Appl Acarol. 2023 Dec;91(4):697-714. doi: 10.1007/s10493-023-00867-3. Epub 2023 Nov 20.
Rhipicephalus sanguineus sensu lato 'tropical lineage' (Acari: Ixodidae) is considered a sanitary concern due to its role as a disease vector. Tick strains resistant to synthetic acaricides have caused difficulties in their control, besides synthetic acaricides are harmful to the environment and to the health of non-target animals. The research of plants with acaricidal and repellent properties has proved to be an efficient alternative in tick control. The genus Tagetes spp. excels for its use as traditional pest control in households and plantations and also for its potential as an acaricide against R. sanguineus under laboratory conditions. The first aim of the present study was to evaluate the effect of different doses of Tagetes minuta essential oil (TMEO) on the central nervous system (synganglion) in unfed R. sanguineus adults. The histological analysis of synganglion exposed to the different concentrations of TMEO and amitraz 12.5% (50% of the recommended dose in the package insert) showed a significant effect with signs of cell damage including volume increase, loss of shape, and vacuolization, in addition to chromatin alterations such as condensation, margination, and fragmentation. TMEO were analyzed by gas chromatography coupled with mass spectrometry showing the presence of 21 compounds that according to their chemical structure are classified as terpenoids. Among them (Z)-β-ocimene, ocimene, (Z)-tagetone, and verbenone were found in major quantities.
传播疾病的“热带谱系”（蜱螨目：硬蜱科）的血革蜱（Rhipicephalus sanguineus sensu lato）因其作为疾病载体而被认为是一种卫生关注点。对合成杀蜱剂具有抗药性的蜱虫株在控制方面造成了困难，此外，合成杀蜱剂对环境和非目标动物的健康有害。具有杀蜱和驱虫特性的植物研究已被证明是控制蜱虫的有效替代方法。万寿菊属（Tagetes spp.）因其在家庭和种植园中作为传统害虫防治的用途以及在实验室条件下作为防治血革蜱的杀蜱剂的潜力而表现出色。本研究的首要目标是评估不同剂量的小花万寿菊精油（TMEO）对未进食的血革蜱成虫中枢神经系统（神经节）的影响。暴露于不同浓度的 TMEO 和 12.5%甲脒（标签上推荐剂量的 50%）的神经节的组织学分析显示出显著的效果，表现为细胞损伤的迹象，包括体积增加、形状丧失和空泡化，以及染色质改变，如浓缩、边缘化和碎片化。通过气相色谱-质谱联用分析 TMEO，发现了 21 种化合物，根据其化学结构可分为萜类化合物。其中（Z）-β-罗勒烯、罗勒烯、（Z）-香叶烯和马鞭草烯酮的含量较高。
