Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, 501 North Columbia Road, Stop 9061, Grand Forks, ND, 58202, USA.
Sci Rep. 2023 Nov 20;13(1):20320. doi: 10.1038/s41598-023-47691-8.
Metabolism, known to be temporally regulated to meet evolving energy demands, plays a crucial role in shaping developmental pace. Recent studies have demonstrated that two key proteins PARP1 and PARG play a regulatory role in the transcription of both morphogenic and metabolic genes. Intriguingly, in Drosophila, the depletion of PARP1 or PARG proteins causes a developmental arrest before pupation, resulting in individuals unable to complete their development. This phenotype highlights the critical involvement of poly(ADP-ribosyl)ating enzymes in regulating the metamorphic process. In this study, we provide compelling evidence that these enzymes intricately coordinate transcriptional changes in both developmental and metabolic pathways during metamorphosis. Specifically, they promote the expression of genes crucial for pupation, while simultaneously negatively regulating the expression of metabolic genes before the transition to the pupal stage. Additionally, these enzymes suppress the expression of genes that are no longer required during this transformative period. Our findings shed light on the intricate interplay between poly(ADP-ribosyl)ating enzymes, developmental processes, and metabolic regulation before metamorphosis and highlight a new role of poly(ADP-ribosyl)ating enzymes in the global regulation of transcription.
代谢，已知是时间调节以满足不断变化的能量需求，在塑造发育速度方面起着关键作用。最近的研究表明，两种关键蛋白 PARP1 和 PARG 在形态发生和代谢基因的转录中起调节作用。有趣的是，在果蝇中，PARP1 或 PARG 蛋白的耗竭会导致蛹化前的发育停滞，导致个体无法完成发育。这种表型突出了聚（ADP-核糖）化酶在调节变态过程中的关键作用。在这项研究中，我们提供了令人信服的证据，证明这些酶在变态过程中错综复杂地协调了发育和代谢途径中的转录变化。具体来说，它们促进了蛹化所需基因的表达，同时在向蛹期过渡之前负调节代谢基因的表达。此外，这些酶抑制了在这个转型期不再需要的基因的表达。我们的发现揭示了聚（ADP-核糖）化酶、发育过程和变态前代谢调节之间的复杂相互作用，并强调了聚（ADP-核糖）化酶在转录全局调节中的新作用。
