State key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, China.
Yunnan Provincial Key Laboratory of Molecular Biology for Sinomedicine, Yunnan University of Traditional Chinese Medicine, Kunming, China.
Elife. 2023 Nov 21;12:e85362. doi: 10.7554/eLife.85362.
An imbalance of the gut microbiota, termed dysbiosis, has a substantial impact on host physiology. However, the mechanism by which host deals with gut dysbiosis to maintain fitness remains largely unknown. In , , which is its bacterial diet, proliferates in its intestinal lumen during aging. Here, we demonstrate that progressive intestinal proliferation of activates the transcription factor DAF-16, which is required for maintenance of longevity and organismal fitness in worms with age. DAF-16 up-regulates two lysozymes and , thus limiting the bacterial accumulation in the gut of worms during aging. During dysbiosis, the levels of indole produced by are increased in worms. Indole is involved in the activation of DAF-16 by TRPA-1 in neurons of worms. Our finding demonstrates that indole functions as a microbial signal of gut dysbiosis to promote fitness of the host.
肠道微生物群落失衡，即所谓的生态失调，对宿主生理有重大影响。然而，宿主如何应对肠道生态失调以维持健康仍然很大程度上未知。在衰老过程中，其细菌饮食在肠道腔中大量增殖。在这里，我们证明 肠道的逐渐增殖激活了转录因子 DAF-16，这对于维持老龄蠕虫的长寿和机体健康是必需的。DAF-16 上调了两种溶菌酶 和 ，从而限制了老龄蠕虫肠道中细菌的积累。在生态失调期间，由 产生的吲哚水平在蠕虫中增加。吲哚参与了蠕虫神经元中 TRPA-1 对 DAF-16 的激活。我们的发现表明，吲哚作为肠道生态失调的微生物信号，促进宿主的健康。
