Department of Effect-Directed Analysis, Helmholtz Centre for Environmental Research - UFZ, Leipzig 04318, Germany.
Department of Cell Toxicology, Helmholtz Centre for Environmental Research - UFZ, Leipzig 04318, Germany.
Environ Sci Technol. 2023 Dec 5;57(48):19363-19373. doi: 10.1021/acs.est.3c05962. Epub 2023 Nov 21.
Human biomonitoring studies are important for understanding adverse health outcomes caused by exposure to chemicals. Complex mixtures of chemicals detected in blood - the blood exposome - may serve as proxies for systemic exposure. Ideally, several analytical methods are combined with bioassays to capture chemical mixtures as diverse as possible. How many and which (bio)analyses can be performed is limited by the sample volume and compatibility of extraction and (bio)analytical methods. We compared the extraction efficacy of three extraction methods using pooled human plasma spiked with >400 organic chemicals. Passive equilibrium sampling (PES) with polydimethylsiloxane (PDMS) followed by solid phase extraction (PES + SPE), SPE alone (SPE), and solvent precipitation (SolvPrec) were compared for chemical recovery in LC-HRMS and GC-HRMS as well as effect recovery in four mammalian cell lines (AhR-CALUX, SH-SY5Y, AREc32, PPARγ-BLA). The mean chemical recoveries were 38% for PES + SPE, 27% for SPE, and 61% for SolvPrec. PES + SPE enhanced the mean chemical recovery compared to SPE, especially for neutral hydrophobic chemicals. PES + SPE and SolvPrec had effect recoveries of 100-200% in all four cell lines, outperforming SPE, which had 30-100% effect recovery. Although SolvPrec has the best chemical recoveries, it does not remove matrix like inorganics or lipids, which might pose problems for some (bio)analytical methods. PES + SPE is the most promising method for sample preparation in human biomonitoring as it combines good recoveries with cleanup, enrichment, and potential for high throughput.
人体生物监测研究对于了解化学物质暴露引起的不良健康后果非常重要。血液中检测到的化学物质的复杂混合物 - 血液暴露组 - 可以作为全身暴露的替代物。理想情况下，将几种分析方法与生物测定法结合使用，以尽可能多地捕获化学混合物。可以进行多少种和哪种（生物）分析受到样品体积和提取和（生物）分析方法的兼容性的限制。我们比较了三种提取方法在含有>400 种有机化学物质的混合人血浆中的提取效果。使用聚二甲基硅氧烷（PDMS）进行被动平衡采样（PES），然后进行固相萃取（PES + SPE），单独进行固相萃取（SPE）和溶剂沉淀（SolvPrec），比较了 LC-HRMS 和 GC-HRMS 中的化学回收率以及四种哺乳动物细胞系（AhR-CALUX、SH-SY5Y、AREc32、PPARγ-BLA）中的效应回收率。PES + SPE 的平均化学回收率为 38%，SPE 为 27%，SolvPrec 为 61%。与 SPE 相比，PES + SPE 提高了平均化学回收率，特别是对于中性疏水性化学物质。PES + SPE 和 SolvPrec 在所有四种细胞系中的效应回收率均为 100-200%，优于 SPE 的 30-100%。尽管 SolvPrec 具有最佳的化学回收率，但它不能去除无机物或脂质等基质，这可能会对某些（生物）分析方法造成问题。PES + SPE 是人体生物监测中最有前途的样品制备方法，因为它结合了良好的回收率、净化、富集和高通量的潜力。
