Arthropod Genetics, The Pirbright Institute, Ash Road, Pirbright, GU24 0HN, UK.
Department of Biology, University of York, Heslington, YO10 5DD, UK.
Sci Rep. 2023 Nov 21;13(1):20352. doi: 10.1038/s41598-023-47777-3.
Molecular tools for modulating transgene expression in Aedes aegypti are few. Here we demonstrate that adjustments to the AePUb promoter length can alter expression levels of two reporter proteins in Ae. aegypti cell culture and in mosquitoes. This provides a simple means for increasing or decreasing expression of a gene of interest and easy translation from cells to whole insects.
用于调节埃及伊蚊中转基因表达的分子工具很少。在这里,我们证明 AePUb 启动子长度的调整可以改变 Ae. aegypti 细胞培养和蚊子中两种报告蛋白的表达水平。这为增加或减少感兴趣基因的表达提供了一种简单的方法,并且易于从细胞转化为完整的昆虫。