Hospital de la Solidaridad-Los Olivos, Lima, Perú.
Departamento de Nutrición, Facultad de Medicina, Universidad Nacional Mayor de San Marcos, Lima, Perú.
Rev Peru Med Exp Salud Publica. 2023 Jul-Sep;40(3):317-324. doi: 10.17843/rpmesp.2023.403.12481. Epub 2023 Sep 28.
OBJECTIVE.: To determine the in vitro antioxidant capacity of Corryocactus brevistylus and its effect on glycemia and the pancreas of alloxan-induced diabetic rats.
MATERIALS AND METHODS.: The antioxidant capacity of the hydroethanolic extract of sanky (HEES) was evaluated by assessing its ability to reduce 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric ion (FRAP). We used thirty adult rats, which were induced to diabetes with two doses of alloxan (80mg/kg). Rats were distributed into 5 groups (n=6), all groups received treatment by orogastric route for eight days. Group I received water, group II received metformin 14mg/kg and groups III, IV and V received sanky juice at 1.0; 4.0 and 16 mL/kg, respectively. Glycemia was evaluated by the rapid method (glucometer) (first and eighth day). After treatment, the animals were sacrificed and the pancreas was removed for histopathological study.
RESULTS.: The antioxidant capacity of HEES by DPPH showed an IC50 of 0.77 mg/mL; the FRAP method showed a TEAC-FRAP of 22.31µg/mg. Glycemia decreased on the eighth day of treatment, with respect to the first day; a decrease in glycemia was also found in groups III-V, when compared to group I. Histologically, groups I-II presented severe atrophy and moderate necrosis of the islets of Langerhans; groups IV-V presented hypertrophy and mild multifocal necrosis at the islet level.
CONCLUSIONS.: The extract of sanky showed antioxidant capacity in vitro and the juice exerts a hypoglycemic and protective effect on the pancreas.
测定 Corryocactus brevistylus 的体外抗氧化能力及其对血糖和丙烯醛诱导糖尿病大鼠胰腺的影响。
通过评估其还原 2,2-二苯基-1-苦基肼（DPPH）和铁离子（FRAP）的能力来评估水醇提取物（HEES）的抗氧化能力。我们使用了 30 只成年大鼠，用两剂量丙烯醛（80mg/kg）诱导其发生糖尿病。大鼠分为 5 组（n=6），所有组均通过口服途径给药 8 天。第 1 组给予水，第 2 组给予二甲双胍 14mg/kg，第 3、4 和 5 组分别给予 sanky 汁 1.0、4.0 和 16mL/kg。第 1 天和第 8 天通过快速方法（血糖仪）评估血糖。治疗后，处死动物并取出胰腺进行组织病理学研究。
HEES 通过 DPPH 的抗氧化能力的 IC50 为 0.77mg/mL；FRAP 法显示 TEAC-FRAP 为 22.31µg/mg。与第 1 天相比，治疗第 8 天血糖降低；与第 1 组相比，第 3-5 组的血糖也有所降低。组织学上，第 1-2 组胰岛严重萎缩，中度坏死；第 4-5 组胰岛水平呈现肥大和轻度多灶性坏死。
sanky 的提取物具有体外抗氧化能力，果汁对胰腺具有降血糖和保护作用。
