Department of Microbiology, Faculty of Science, Chulalongkorn University, Patumwan, Bangkok, Thailand.
Department of Materials Science, Faculty of Science, Chulalongkorn University, Patumwan, Bangkok, Thailand.
PLoS One. 2023 Nov 22;18(11):e0292051. doi: 10.1371/journal.pone.0292051. eCollection 2023.
The aim of this study was to utilize cassava pulp to prepare biocomposites comprising microcrystalline cellulose from cassava pulp (CP-MCC) as a filler and polyhydroxybutyrate (PHB) synthesized in-house by Cupriavidus necator strain A-04. The CP-MCC was extracted from fresh cassava pulp. Next, the CP-MCC surface was modified with butyryl chloride (esterified to CP-MCC butyrate) to improve dissolution and compatibility with the PHB. FTIR results confirmed that the esterified CP-MCC butyrate had aliphatic chains replacing the hydroxyl groups; this substitution increased the solubilities in acetone, chloroform, and tetrahydrofuran. Biocomposite films were prepared by varying the composition of esterified CP-MCC butyrate as a filler in the PHB matrix at 0, 5, 10, 15, 20 and 100 wt%. The results for the 95:5 and 90:10 CP-MCC butyrate biocomposite films showed that esterification led to improvements in the thermal properties and increased tensile strengths and elongations at break. All prepared biocomposite films maintained full biodegradability.
本研究旨在利用木薯渣制备生物复合材料，其中包含木薯渣微晶纤维素（CP-MCC）作为填充剂和由 Cupriavidus necator 菌株 A-04 合成的聚羟基丁酸酯（PHB）。CP-MCC 是从新鲜的木薯渣中提取的。接下来，CP-MCC 表面用丁酰氯进行修饰（酯化到 CP-MCC 丁酸酯上），以提高其在 PHB 中的溶解性和相容性。傅里叶变换红外光谱（FTIR）结果证实，酯化的 CP-MCC 丁酸酯具有取代羟基的脂肪族链；这种取代增加了在丙酮、氯仿和四氢呋喃中的溶解度。通过在 PHB 基质中以 0、5、10、15、20 和 100wt%的比例变化酯化 CP-MCC 丁酸酯作为填充剂来制备生物复合材料薄膜。对于 95:5 和 90:10 CP-MCC 丁酸酯生物复合材料薄膜的结果表明，酯化导致热性能提高，拉伸强度和断裂伸长率增加。所有制备的生物复合材料薄膜均保持完全可生物降解性。
