Yangon Central Women's Hospital, Yangon, Myanmar; West Yangon General Hospital, Yangon, Myanmar.
Department of Microbiology and Immunology, School of Biomedical Sciences, University of Otago, Dunedin, New Zealand.
J Glob Antimicrob Resist. 2024 Mar;36:1-3. doi: 10.1016/j.jgar.2023.11.004. Epub 2023 Nov 20.
Antimicrobial resistance (AMR) including multidrug-resistant (MDR) and extensively drug-resistant (XDR) Pseudomonas aeruginosa has emerged as one of the serious public health threats across the globe. Southeast Asia is a 'hot spot' of antimicrobial-resistant bacteria, including MDR P. aeruginosa. Despite Myanmar being located in Southeast Asia and suffering from a high infectious disease burden, data on MDR and XDR P. aeruginosa from Myanmar are limited. In this communication, we report the draft genome of an XDR P. aeruginosa isolate, MMXDRPA001, that was identified during a routine diagnosis in Myanmar.
An MMXDRPA001 isolate colonising a hospitalised patient was characterised by antibiotic resistance profiling following standard methods and whole-genome sequencing using an Illumina MiSeq platform. The generated reads were de novo assembled using SPAdes (v.3.9.1). Annotation was performed by Prokka (v.1.14.0). Sequence type, antimicrobial resistance and virulence-related genes were predicted from the sequence. The phylogenetic relationships of all P. aeruginosa isolates were determined using core genome single-nucleotide polymorphisms (SNPs) analysis utilising Snippy (v.4.6.0) and Gubbins (v.2.3.4).
P. aeruginosa MMXDRPA001 was resistant to most antipseudomonal β-lactams, aminoglycosides and quinolones. The assembly comprised 145 contigs totalling 6 808 493 bases of sequence and a total of 6183 coding sequences. The isolate belonged to sequence type (ST) 235, contained carbapenemase-encoding gene bla and was clonally related to a previously reported isolate from Thailand.
The identification of an international high-risk clone of ST235 XDR isolate in Myanmar, genomically relating to that from a neighbouring country underscores the need for coordinated AMR surveillance throughout healthcare settings in Myanmar and in the Southeast Asia region.
包括多药耐药（MDR）和广泛耐药（XDR）铜绿假单胞菌在内的抗微生物药物耐药性（AMR）已成为全球范围内严重的公共卫生威胁之一。东南亚是抗菌药物耐药细菌的“热点”地区，包括 MDR 铜绿假单胞菌。尽管缅甸位于东南亚，且传染病负担高，但缅甸有关 MDR 和 XDR 铜绿假单胞菌的数据有限。在本通讯中，我们报告了在缅甸常规诊断中发现的一株 XDR 铜绿假单胞菌分离株 MMXDRPA001 的基因组草图。
采用标准方法对定植于住院患者的 MMXDRPA001 分离株进行抗生素耐药谱特征分析，并使用 Illumina MiSeq 平台进行全基因组测序。使用 SPAdes（v.3.9.1）对生成的reads 进行从头组装。使用 Prokka（v.1.14.0）进行注释。从序列中预测序列类型、抗微生物药物耐药性和毒力相关基因。利用 Snippy（v.4.6.0）和 Gubbins（v.2.3.4）利用核心基因组单核苷酸多态性（SNP）分析确定所有铜绿假单胞菌分离株的系统发育关系。
铜绿假单胞菌 MMXDRPA001 对大多数抗假单胞菌β-内酰胺类、氨基糖苷类和喹诺酮类药物耐药。组装由 145 个 contigs 组成，总序列为 6808493 个碱基，共包含 6183 个编码序列。该分离株属于序列型（ST）235，携带碳青霉烯酶基因 bla，与来自泰国的先前报道的分离株在克隆上相关。
在缅甸发现的一株国际高风险克隆 ST235 XDR 分离株，其基因组与邻国的分离株相关，这凸显了在缅甸和东南亚地区整个医疗机构中协调开展抗微生物药物耐药性监测的必要性。
