Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
Department of Clinical Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil.
J Glob Antimicrob Resist. 2018 Mar;12:88-89. doi: 10.1016/j.jgar.2017.12.009. Epub 2017 Dec 22.
The incidence of multidrug-resistant bacteria in wildlife animals has been investigated to improve our knowledge of the spread of clinically relevant antimicrobial resistance genes. The aim of this study was to report the first draft genome sequence of an extensively drug-resistant (XDR) Pseudomonas aeruginosa ST644 isolate recovered from a Magellanic penguin with a footpad infection (bumblefoot) undergoing rehabilitation process.
The genome was sequenced on an Illumina NextSeq platform using 150-bp paired-end reads. De novo genome assembly was performed using Velvet v.1.2.10, and the whole genome sequence was evaluated using bioinformatics approaches from the Center of Genomic Epidemiology, whereas an in-house method (mapping of raw whole genome sequence reads) was used to identify chromosomal point mutations.
The genome size was calculated at 6436450bp, with 6357 protein-coding sequences and the presence of genes conferring resistance to aminoglycosides, β-lactams, phenicols, sulphonamides, tetracyclines, quinolones and fosfomycin; in addition, mutations in the genes gyrA (Thr83Ile), parC (Ser87Leu), phoQ (Arg61His) and pmrB (Tyr345His), conferring resistance to quinolones and polymyxins, respectively, were confirmed.
This draft genome sequence can provide useful information for comparative genomic analysis regarding the dissemination of clinically significant antibiotic resistance genes and XDR bacterial species at the human-animal interface.
研究野生动物中多药耐药菌的发生率，以提高我们对临床相关抗菌耐药基因传播的认识。本研究旨在报告从一只正在康复过程中患有足底感染（蹠球炎）的麦哲伦企鹅中分离出的一株广泛耐药（XDR）铜绿假单胞菌 ST644 分离株的全基因组序列草案。
使用 150-bp 配对末端读取在 Illumina NextSeq 平台上对基因组进行测序。使用 Velvet v.1.2.10 进行从头基因组组装，使用基因组流行病学中心的生物信息学方法评估全基因组序列，而内部方法（原始全基因组序列读取的映射）用于鉴定染色体点突变。
基因组大小计算为 6436450bp，包含 6357 个编码蛋白的序列和赋予对氨基糖苷类、β-内酰胺类、苯氧青霉素类、磺胺类、四环素类、喹诺酮类和磷霉素耐药的基因；此外，还证实了分别赋予喹诺酮类和多粘菌素耐药的 gyrA（Thr83Ile）、parC（Ser87Leu）、phoQ（Arg61His）和 pmrB（Tyr345His）基因中的突变。
该草案基因组序列可为人类-动物界面临床重要抗生素耐药基因和 XDR 细菌种属传播的比较基因组分析提供有用信息。
