Abd-El-Haleem Desouky
Environmental Biotechnology Department, Genetic Engineering and Biotechnology Institute, City of Scientific Research and Technological Applications, Burgelarab, Alexandria, 21934, Egypt.
J Genet Eng Biotechnol. 2023 Nov 22;21(1):133. doi: 10.1186/s43141-023-00609-3.
This study identified an extracellular bacterial polysaccharide produced by Bacillus velezensis strain 40B that contains more than 90% of the monosaccharide glucose as alpha-glucan. A prominent peak at 1074 cm, a characteristic of glycoside couplings, was visible in the FTIR spectrum. There were traces of xylose, sucrose, and lactose, according to the HPLC study. The ability of this bacterial glucan to operate as a biosorbent of the heavy metals cobalt, chromium, copper, and lead from aqueous solutions was investigated in conjunction with Ca-alginate beads. It proved that glucan 40B has a low affinity for chromium ions and is selective for lead. Initial concentration measurements showed an inverse relationship between concentration and the amount of metal ions eliminated. Lead and chromium removal increased as the glucan dose was increased. It was shown that as the pH of the starting solution is elevated, there is an increase in the sorption of metal ions onto the glucan. It was clear that when the temperature increased, the fraction of metal ion sorption slightly increased. Glucan has a wide range of industrial applications, from food and medicine to health and nutrition. As a result, the investigation's scope was expanded to include heavy metal removal.
本研究鉴定出一种由解淀粉芽孢杆菌40B菌株产生的细胞外细菌多糖,其含有超过90%的单糖葡萄糖,以α-葡聚糖形式存在。在傅里叶变换红外光谱(FTIR)中,1074 cm处出现一个突出峰,这是糖苷偶联的特征。根据高效液相色谱(HPLC)研究,存在木糖、蔗糖和乳糖的痕迹。结合海藻酸钙珠,研究了这种细菌葡聚糖作为水溶液中重金属钴、铬、铜和铅的生物吸附剂的能力。结果证明,葡聚糖40B对铬离子的亲和力较低,对铅具有选择性。初始浓度测量表明,浓度与去除的金属离子量之间呈反比关系。随着葡聚糖剂量的增加,铅和铬的去除量增加。结果表明,随着起始溶液pH值的升高,金属离子在葡聚糖上的吸附增加。很明显,当温度升高时,金属离子吸附比例略有增加。葡聚糖在食品、医药、健康和营养等领域具有广泛的工业应用。因此,该研究的范围扩大到包括重金属去除。