Ladera Gómez Marla Eliana, Nieto Farias María Victoria, Vater Adrián, Ceriani María Carolina, Dolcini Guillermina Laura
FCV-UNCPBA, CIVETAN (CONICET-UNCPBA-CICPBA).
Escuela de Educación Secundaria Agraria Nº1 "Dr. Ramón Santamarina".
Vet Ital. 2023 Mar 31;59(1):83-92. doi: 10.12834/VetIt.2718.20143.3.
The retrovirus bovine leukemia virus (BLV) might produce abnormal immune function, associated with susceptibility to developing other infectious diseases, including mastitis. This study aimed to determine the proviral load and cytokines gene expression in peripheral blood mononuclear cells (PMBC) and milk somatic cells (SC) in BLV-infected and non-infected cattle. Of 27 BLV-infected cows in PBMC, 17 (62.96%) had a high proviral load (HPL), and 10 (37.04%) had a low proviral load (LPL). All SC samples had low proviral load (LPL-SC). Higher IFN-γ and IL-10 expression, and lower IL-12 and IL-6 expression, were found in PBMC from BLV-infected compared to BLV non-infected cattle. Moreover, higher IFN-γ, IL-12, and IL-6 expression, and lower IL-10 expression were observed in cattle with LPL-PBMC compared to HPL-PBMC. In milk samples, lower IFN-γ and higher IL-12 mRNA expression were observed in LPL-SC compared to BLV non-infected cattle in SC. IL-10 and IL-6 expression mRNA was significantly lower in LPL-SC than in SC from BLV non-infected cattle. This study shows that milk SC maintains lower proviral load levels than PBMC. This first report on Th1 and Th2 cytokines expression levels in SC may be relevant to future control strategies for BLV infection, mastitis, and udder health management.
逆转录病毒牛白血病病毒（BLV）可能会导致免疫功能异常，这与包括乳腺炎在内的其他传染病易感性有关。本研究旨在确定感染和未感染BLV的牛外周血单个核细胞（PMBC）和乳体细胞（SC）中的前病毒载量和细胞因子基因表达。在27头感染BLV的奶牛的PBMC中，17头（62.96%）前病毒载量高（HPL），10头（37.04%）前病毒载量低（LPL）。所有SC样本前病毒载量均低（LPL-SC）。与未感染BLV的牛相比，感染BLV的牛的PBMC中IFN-γ和IL-10表达较高，而IL-12和IL-6表达较低。此外，与HPL-PBMC的牛相比，LPL-PBMC的牛中观察到更高的IFN-γ、IL-12和IL-6表达，以及更低的IL-10表达。在乳样中，与未感染BLV的牛的SC相比，LPL-SC中IFN-γ较低，IL-12 mRNA表达较高。LPL-SC中IL-10和IL-6表达的mRNA显著低于未感染BLV的牛的SC。本研究表明，乳SC中的前病毒载量水平低于PBMC。这篇关于SC中Th1和Th2细胞因子表达水平的首次报告可能与未来BLV感染、乳腺炎和乳房健康管理的控制策略有关。
