Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250014, China.
Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250014, China.
J Chromatogr A. 2024 Jan 4;1713:464520. doi: 10.1016/j.chroma.2023.464520. Epub 2023 Nov 21.
Aflatoxins (AFs) exhibit hepatotoxicity, immunotoxicity, and carcinogenicity, and their detection in food has attracted widespread concern. An ordered macroporous metal-organic framework (OM-ZIF-8) based on solid-phase extraction (SPE) was used to extract six AFs from milk products. The SPE conditions, including eluting solvent, eluting volume, amounts of OM-ZIF-8, pH of loading solution, loading solvent, ionic strength, loading flow rate, and elution flow rate, were exhaustively optimized. Under optimal parameters, the six AFs were detected by ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The OM-ZIF-8 exhibited satisfactory AFs extraction performance through ordered macropore structure, π-π interaction, coordination interaction, and electrostatic interaction. Furthermore, linearity in the range of 0.01-100 ng mL with low detection limits of 0.002-0.0150 ng mL was obtained, and the relative recoveries of AFs were 80.3-110 % with relative standard deviation ≤8.7 %. Thus, this research provides a promising platform for the analysis of trace AFs in complex foods.
黄曲霉毒素（AFs）具有肝毒性、免疫毒性和致癌性，其在食品中的检测受到广泛关注。本研究采用基于固相萃取（SPE）的有序大孔金属有机骨架（OM-ZIF-8）提取乳制品中的六种 AFs。通过对 SPE 条件（洗脱溶剂、洗脱体积、OM-ZIF-8 用量、上样溶液 pH 值、上样溶剂、离子强度、上样流速和洗脱流速）进行全面优化，实现了对六种 AFs 的检测。在最佳参数下，采用超高效液相色谱-串联质谱法（UHPLC-MS/MS）对 AFs 进行检测。OM-ZIF-8 通过有序大孔结构、π-π 相互作用、配位相互作用和静电相互作用表现出良好的 AFs 提取性能。此外，该方法在 0.01-100ng/mL 范围内具有良好的线性关系，检测限为 0.002-0.0150ng/mL，AFs 的相对回收率为 80.3%-110%，相对标准偏差≤8.7%。因此，本研究为分析复杂食品中痕量 AFs 提供了一个有前景的平台。
