National Key Laboratory of Plant Molecular Genetics, Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China; University of Chinese Academy of Sciences, Beijing 100049, China.
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing 100101, China.
Curr Biol. 2023 Dec 18;33(24):5355-5367.e5. doi: 10.1016/j.cub.2023.10.072. Epub 2023 Nov 22.
Soybean (Glycine max) is a crop with high demand for molybdenum (Mo) and typically requires Mo fertilization to achieve maximum yield potential. However, the genetic basis underlying the natural variation of Mo concentration in soybean and its impact on soybean agronomic performance is still poorly understood. Here, we performed a genome-wide association study (GWAS) to identify GmMOT1.1 and GmMOT1.2 that drive the natural variation of soybean Mo concentration and confer agronomic traits by affecting auxin synthesis. The soybean population exhibits five haplotypes of the two genes, with the haplotype 5 demonstrating the highest expression of GmMOT1.1 and GmMOT1.2, as well as the highest transport activities of their proteins. Further studies showed that GmMOT1.1 and GmMOT1.2 improve soybean yield, especially when cultivated in acidic or slightly acidic soil. Surprisingly, these two genes contribute to soybean growth by enhancing the activity of indole-3-acetaldehyde (IAAld) aldehyde oxidase (AO), leading to increased indole-3-acetic acid (IAA) synthesis, rather than being involved in symbiotic nitrogen fixation or nitrogen assimilation. Furthermore, the geographical distribution of five haplotypes in China and their correlation with soil pH suggest the potential significance of GmMOT1.1 and GmMOT1.2 in soybean breeding strategies.
大豆（Glycine max）是一种对钼（Mo）需求量很高的作物，通常需要 Mo 施肥才能达到最大产量潜力。然而，大豆中钼浓度自然变异的遗传基础及其对大豆农艺性能的影响仍知之甚少。在这里，我们进行了全基因组关联研究（GWAS），以鉴定出 GmMOT1.1 和 GmMOT1.2，它们通过影响生长素合成来驱动大豆钼浓度的自然变异，并赋予农艺性状。大豆群体表现出这两个基因的五种单倍型，其中单倍型 5 表现出最高的 GmMOT1.1 和 GmMOT1.2 表达水平，以及它们蛋白质的最高运输活性。进一步的研究表明，GmMOT1.1 和 GmMOT1.2 提高了大豆的产量，尤其是在酸性或略酸性土壤中种植时。令人惊讶的是，这两个基因通过增强吲哚-3-乙醛（IAAld）醛氧化酶（AO）的活性来促进吲哚-3-乙酸（IAA）的合成，从而促进大豆的生长，而不参与共生固氮或氮同化。此外，中国五个单倍型的地理分布及其与土壤 pH 的相关性表明，GmMOT1.1 和 GmMOT1.2 在大豆育种策略中具有潜在的重要性。
