Howard Hughes Medical Institute and Department of Molecular Biosciences, The University of Texas at Austin, Austin, Texas, USA.
Institute of Transformative Biomolecules, Nagoya University, Nagoya, Japan.
Plant Cell Environ. 2024 Sep;47(9):3288-3298. doi: 10.1111/pce.14761. Epub 2023 Nov 23.
The formation of stomata presents a compelling model system for comprehending the initiation, proliferation, commitment and differentiation of de novo lineage-specific stem cells. Precise, timely and robust cell fate and identity decisions are crucial for the proper progression and differentiation of functional stomata. Deviations from this precise specification result in developmental abnormalities and nonfunctional stomata. However, the molecular underpinnings of timely cell fate commitment have just begun to be unravelled. In this review, we explore the key regulatory strategies governing cell fate commitment, emphasizing the distinctions between embryonic and postembryonic stomatal development. Furthermore, the interplay of transcription factors and cell cycle machineries is pivotal in specifying the transition into differentiation. We aim to synthesize recent studies utilizing single-cell as well as cell-type-specific transcriptomics, epigenomics and chromatin accessibility profiling to shed light on how master-regulatory transcription factors and epigenetic machineries mutually influence each other to drive fate commitment and maintenance.
气孔的形成提供了一个引人注目的模式系统，可用于理解新的谱系特异性干细胞的起始、增殖、定型和分化。精确、及时和稳健的细胞命运和身份决策对于功能性气孔的正常发育和分化至关重要。偏离这种精确的规范会导致发育异常和无功能的气孔。然而，及时的细胞命运决定的分子基础才刚刚开始被揭示。在这篇综述中，我们探讨了控制细胞命运决定的关键调控策略，强调了胚胎期和胚胎后气孔发育之间的区别。此外，转录因子和细胞周期机制的相互作用对于指定向分化的转变至关重要。我们的目标是综合最近的研究，利用单细胞和细胞类型特异性转录组学、表观基因组学和染色质可及性分析，阐明主调控转录因子和表观遗传机制如何相互影响，以驱动命运决定和维持。
