比较外科和结肠镜组织以建立结直肠患者来源的类器官。

Comparison of Surgical and Colonoscopy Tissue to Establish Colorectal Patient-derived Organoids.

机构信息

Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China.

Fujian Key Laboratory of Integrative Medicine in Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China.

出版信息

Curr Cancer Drug Targets. 2024;24(5):546-555. doi: 10.2174/0115680096263866231024112120.

DOI:10.2174/0115680096263866231024112120

PMID:37997804

Abstract

BACKGROUND

Patient-derived organoids (PDOs) are ex vivo models that retain the functions and characteristics of individualized source tissues, including a simulated tumor microenvironment. However, the potential impact of undiscovered differences between tissue sources on PDO growth and progression remains unclear.

OBJECTIVE

This study aimed to compare the growth and condition of PDO models originating from surgical resection and colonoscopy and to provide practical insights for PDO studies.

METHODS

Tissue samples and relevant patient clinical information were collected to establish organoid models. PDOs were derived from both surgical and colonoscopy tissues. The growth of the organoids, including their state, size, and success rate of establishment, was recorded and analyzed. The activity of the organoids at the end stage of growth was detected using calcein-AM fluorescence staining.

RESULTS

The results showed that the early growth phase of 2/3 colonoscopy-derived organoids was faster compared to surgical PDOs, with a growth difference observed within 11-13 days of establishment. However, colonoscopy-derived organoids exhibited a diminished growth trend after this time. There were no significant differences observed in the terminal area and quantity between the two types of tissue-derived organoids. Immunofluorescence assays of the PDOs revealed that the surgical PDOs possessed a denser cell mass with relatively higher viability than colonoscopy-derived PDOs.

CONCLUSION

In the establishment of colorectal patient-derived organoids, surgically derived organoids require a slightly longer establishment period, while colonoscopy-derived organoids should be passaged prior to growth inhibition to preserve organoid viability.

摘要

背景

患者来源的类器官（PDO）是一种体外模型，保留了个体化来源组织的功能和特征，包括模拟肿瘤微环境。然而，组织来源之间未被发现的差异对 PDO 生长和进展的潜在影响尚不清楚。

目的

本研究旨在比较来源于手术切除和结肠镜检查的 PDO 模型的生长和状态，并为 PDO 研究提供实际见解。

方法

收集组织样本和相关患者临床信息以建立类器官模型。PDO 来源于手术和结肠镜组织。记录和分析类器官的生长情况，包括其状态、大小和建立成功率。使用钙黄绿素-AM 荧光染色检测类器官生长末期的活性。

结果

结果表明，2/3 的结肠镜来源类器官的早期生长阶段比手术 PDO 更快，在建立后 11-13 天观察到生长差异。然而，此后结肠镜来源的类器官生长趋势减弱。两种组织来源的类器官在终末面积和数量上没有显著差异。PDO 的免疫荧光分析显示，手术 PDO 具有更密集的细胞团，相对而言具有更高的活力，而结肠镜来源的 PDO 则具有较低的活力。