College of Tea Science, Guizhou University, Guiyang 550025, Guizhou, China.
School of Chemistry and Materials Science, Hunan Agricultural University, Changsha 410128, China.
J Agric Food Chem. 2023 Dec 6;71(48):18999-19009. doi: 10.1021/acs.jafc.3c05843. Epub 2023 Nov 24.
contains numerous glycosylated secondary metabolites that provide various benefits to plants and humans. However, the genes that catalyze the glycosylation of multitype metabolites in tea plants remain unclear. Here, 180 uridine diphosphate-dependent glycosyltransferases that may be involved in the biosynthesis of glycosylated secondary metabolites were identified from the National Center for Biotechnology Information public databases. Subsequently, 741 was screened through phylogenetic analysis and gene expression profiling. Compositional and induced expression analyses revealed that 741 was highly expressed in tea tender shoots and was induced under biotic and abiotic stress conditions. enzymatic assays revealed that rCsUGT74Y1 encoded a multifunctional UGT that catalyzed the glycosylation of flavonoids, phenolic acids, lignins, and auxins. Furthermore, 741-overexpressing exhibited enhanced growth and accumulation of flavonol and auxin glucosides. Our findings provide insights into identifying specific UGTs and demonstrate that 741 is a multifunctional UGT that promotes plant development.
富含多种糖基化次生代谢产物，这些产物为植物和人类提供了各种益处。然而，催化茶树中多种代谢物糖基化的基因仍不清楚。在这里，从国家生物技术信息中心公共数据库中鉴定出了 180 种可能参与糖基化次生代谢物生物合成的尿苷二磷酸依赖性糖基转移酶。随后，通过系统发育分析和基因表达谱筛选了 741 种。组成和诱导表达分析表明，741 在茶嫩梢中高表达，并在生物和非生物胁迫条件下诱导表达。酶促分析表明，rCsUGT74Y1 编码一种多功能 UGT，可催化类黄酮、酚酸、木质素和生长素的糖基化。此外，741 过表达的 表现出增强的黄酮醇和生长素糖苷的生长和积累。我们的研究结果为鉴定特定的 UGT 提供了新的见解，并表明 741 是一种促进植物发育的多功能 UGT。
