Tang Yinghong, Tang Huijuan, Zhao Cancai, Liu Fang, Luan Mingbao, Chen Jianrong
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China.
College of Life and Environmental Science, Hunan University of Arts and Science, Changde 415000, China.
Plants (Basel). 2025 Aug 13;14(16):2517. doi: 10.3390/plants14162517.
Lignins and flavonoids, which are derived from the phenylpropanoid pathway and share common precursors, play an important role in (ramie). Uridine diphosphate-glycosyltransferases (UGTs) are essential for the glycosylation of secondary metabolites and are involved in plant growth and stress responses. Hence, this study aimed to screen candidate related to lignin/flavonoid glycosylation and stress responses. A total of 84 s were identified, and all s contain a conserved PGPS domain. Phylogenetic analysis suggested that 10, 5, 1, and 1 putative s might be associated with lignin glycosylation, flavonoid glycosylation, and adverse stress, respectively. Further analysis showed that expression was upregulated and showed a significant positive correlation with lignin content in the phloem and leaf, reaching up to 710 in the xylem after 75 days of germination. expression (in the leaf and xylem) and expression (in the xylem) were upregulated and showed a significant positive correlation with lignin and flavonoid content. In the phloem, expression was downregulated and showed a significant negative correlation with lignin content. expression was upregulated in the stem and leaf under Cd treatment. Overall, we successfully identified four candidate (, , and ); these findings provide insight into the glycosylation mechanisms of lignins and flavonoids and stress responses in ramie.
木质素和类黄酮源自苯丙烷途径且具有共同的前体，在（苎麻）中发挥重要作用。尿苷二磷酸糖基转移酶（UGTs）对于次生代谢物的糖基化至关重要，并参与植物生长和应激反应。因此，本研究旨在筛选与木质素/类黄酮糖基化及应激反应相关的候选（基因）。共鉴定出84个（基因），且所有（基因）均含有一个保守的PGPS结构域。系统发育分析表明，分别有10个、5个、1个和1个推定（基因）可能与木质素糖基化、类黄酮糖基化及逆境胁迫相关。进一步分析表明，（某个基因）表达上调，且与韧皮部和叶片中的木质素含量呈显著正相关，萌发75天后在木质部中高达710。（另一个基因）表达（在叶片和木质部中）和（又一个基因）表达（在木质部中）上调，且与木质素和类黄酮含量呈显著正相关。在韧皮部中，（还有一个基因）表达下调，且与木质素含量呈显著负相关。在镉处理下，（某个基因）在茎和叶中表达上调。总体而言，我们成功鉴定出四个候选（基因）（分别为、、和）；这些发现为苎麻中木质素和类黄酮的糖基化机制及应激反应提供了见解。
