Luo Gang, Gong Ruiguang, Ai Yaotian, Zhu Tongyan, Ren Zhanjun
College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China.
College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350000, China.
Biology (Basel). 2023 Nov 17;12(11):1448. doi: 10.3390/biology12111448.
Hair follicle development directly affects the development of the rabbit fur industry. The growth and development of a hair follicle is modified and regulated by many genes and mechanisms. MA is an important RNA modification. However, there are few studies on the effects of the regulation of mA on hair follicle growth and development. In this study, hematoxylin-eosin (HE) staining was used to explore the difference in hair follicle development between Rex rabbits and Hycole rabbits, and we performed mA sequencing to identify the key genes with mA modification in hair follicle growth. The results showed that the hair length, coarse hair percentage, primary hair follicle ratio, and skin thickness of Hycole rabbits were significantly higher than those of Rex rabbits. However, the proportion of secondary hair follicles in Hycole rabbits was significantly lower than that in Rex rabbits. In addition, we found five differential methylases, 20 differential genes, and 24 differential signaling pathways related to hair growth and development. The results of the Sankey diagram showed that 12 genes were related to 13 signal pathways. Finally, we found that five methylases regulated the development of hair follicles through differential genes/signal pathways. These findings laid a molecular foundation for the function of mA modification in hair development.
毛囊发育直接影响兔毛产业的发展。毛囊的生长发育受到多种基因和机制的调控。N6-甲基腺嘌呤（m⁶A）是一种重要的RNA修饰。然而，关于m⁶A调控对毛囊生长发育影响的研究较少。在本研究中，采用苏木精-伊红（HE）染色法探究獭兔和哈白兔毛囊发育的差异，并进行m⁶A测序以鉴定毛囊生长中发生m⁶A修饰的关键基因。结果表明，哈白兔的毛长、粗毛率、初级毛囊比例和皮肤厚度均显著高于獭兔。然而，哈白兔次级毛囊的比例显著低于獭兔。此外，我们发现了5种差异甲基化酶、20个差异基因和24条与毛发生长发育相关的差异信号通路。桑基图结果显示，12个基因与13条信号通路相关。最后，我们发现5种甲基化酶通过差异基因/信号通路调控毛囊发育。这些发现为m⁶A修饰在毛发发育中的功能奠定了分子基础。
