The Characterization and Differential Analysis of mA Methylation in Hycole Rabbit Muscle and Adipose Tissue and Prediction of Regulatory Mechanism about Intramuscular Fat.

N6-methyladenosine (mA) widely participates in various life processes of animals, including disease, memory, growth and development, etc. However, there is no report on mA regulating intramuscular fat deposition in rabbits. In this study, mA modification of Hycole rabbit muscle and adipose tissues were detected by MeRIP-Seq. In this case, 3 methylases and 12 genes modified by mA were found to be significantly different between muscle and adipose tissues. At the same time, we found 3 methylases can regulate the expression of 12 genes in different ways and the function of 12 genes is related to fat deposition base on existing studies. 12 genes were modified by mA methylase in rabbit muscle and adipose tissues. These results suggest that 3 methylases may regulate the expression of 12 genes through different pathways. In addition, the analysis of results showed that 6 of the 12 genes regulated eight signaling pathways, which regulated intramuscular fat deposition. RT-qPCR was used to validate the sequencing results and found the expression results of RT-qPCR and sequencing results are consistent. In summary, , and regulated intramuscular fat by mA modified gene/signaling pathways. Our work provided a new molecular basis and a new way to produce rabbit meat with good taste.