Yue Zhengkai, Li Chenyang, Liu Yongxu, Liu Mengqi, Zhao Man, Li Fuchang, Liu Lei
Department of Animal Science, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Taian, China.
Qingdao Kangda Food Co., Ltd., Qingdao, China.
J Sci Food Agric. 2022 Apr;102(6):2291-2299. doi: 10.1002/jsfa.11567. Epub 2021 Oct 21.
Rex rabbits are important fur rabbits. Heat stress severely reduces the fur quality of Rex rabbits. The aim of this study was to experimentally investigate the effect of dietary vitamin A (VA) addition on hair follicle development and related signal pathways in Rex rabbits under heat stress.
In the experiment, 90 Rex rabbits were randomly divided into three groups: control group (20-25 °C, fed basic diet), heat stress group (30-34 °C, fed basic diet), and heat stress + VA group (20-25 °C, fed 12 000 IU/kg VA in addition to the basic diet). VA could significantly increase the hair follicle density (P < 0.01), hair length (P < 0.05), and the ratio of secondary to primary hair follicles (P < 0.05). In addition, VA could significantly inhibit the expression of BMP2, BMP4, FGF5, TGF-β1, and miR-214 in heat-stressed Rex rabbits and significantly increase the expression of noggin, IGF1, IGF1R, Wnt10b, CTNNB1, SHH, and miR-203 and the levels of Wnt10b and p-β-catenin; however, there was no significant effect of VA on the expression of EGF and miR-205.
The dietary addition of VA can increase the hair follicle density and fur quality of heat-stressed Rex rabbits. Wnt10/β-catenin, insulin-like growth factor 1 (IGF1), fibroblast growth factor 5 (FGF5), noggin-BMP, and sonic hedgehog (SHH) signaling were associated with VA regulation under heat stress. It is possible that miR-205 and miR-194 contribute to the regulation of Wnt10/β-catenin and bone morphogenetic protein (BMP) signaling. © 2021 Society of Chemical Industry.
獭兔是重要的皮用兔。热应激会严重降低獭兔的毛皮质量。本研究旨在通过实验探究日粮中添加维生素A（VA）对热应激獭兔毛囊发育及相关信号通路的影响。
实验中，90只獭兔随机分为三组：对照组（20 - 25°C，饲喂基础日粮）、热应激组（30 - 34°C，饲喂基础日粮）和热应激 + VA组（20 - 25°C，在基础日粮基础上额外添加12 000 IU/kg VA）。VA可显著增加毛囊密度（P < 0.01）、毛长（P < 0.05）以及次级毛囊与初级毛囊的比例（P < 0.05）。此外，VA可显著抑制热应激獭兔中BMP2、BMP4、FGF5、TGF-β1和miR - 214的表达，并显著增加noggin、IGF1、IGF1R、Wnt10b、CTNNB1、SHH和miR - 203的表达以及Wnt10b和p - β - 连环蛋白的水平；然而，VA对EGF和miR - 205的表达无显著影响。
日粮中添加VA可增加热应激獭兔的毛囊密度和毛皮质量。在热应激条件下，Wnt10/β - 连环蛋白、胰岛素样生长因子1（IGF1）、成纤维细胞生长因子5（FGF5）、noggin - BMP和音猬因子（SHH）信号通路与VA的调节有关。miR - 205和miR - 194可能参与Wnt10/β - 连环蛋白和骨形态发生蛋白（BMP）信号通路的调节。© 2021化学工业协会。
