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使用计算光谱学和固态探测器的高灵敏度单线态氧发光传感器。

High Sensitivity Singlet Oxygen Luminescence Sensor Using Computational Spectroscopy and Solid-State Detector.

作者信息

Yu Tiffany C, Davis Steve J, Scimone Mark T, Grimble John, Maguluri Gopi, Anand Sanjay, Cheng Cheng-En, Maytin Edward, Cao Xu, Pogue Brian W, Zhao Youbo

机构信息

Physical Sciences Inc., Andover, MA 01810, USA.

Cleveland Clinic, Cleveland, OH 44195, USA.

出版信息

Diagnostics (Basel). 2023 Nov 12;13(22):3431. doi: 10.3390/diagnostics13223431.

DOI:10.3390/diagnostics13223431
PMID:37998567
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10670281/
Abstract

This paper presents a technique for high sensitivity measurement of singlet oxygen luminescence generated during photodynamic therapy (PDT) and ultraviolet (UV) irradiation on skin. The high measurement sensitivity is achieved by using a computational spectroscopy (CS) approach that provides improved photon detection efficiency compared to spectral filtering methodology. A solid-state InGaAs photodiode is used as the CS detector, which significantly reduces system cost and improves robustness compared to photomultiplier tubes. The spectral resolution enables high-accuracy determination and subtraction of photosensitizer fluorescence baseline without the need for time-gating. This allows for high sensitivity detection of singlet oxygen luminescence emission generated by continuous wave light sources, such as solar simulator sources and those commonly used in PDT clinics. The value of the technology is demonstrated during in vivo and ex vivo experiments that show the correlation of measured singlet oxygen with PDT treatment efficacy and the illumination intensity on the skin. These results demonstrate the potential use of the technology as a dosimeter to guide PDT treatment and as an analytical tool supporting the development of improved sunscreen products for skin cancer prevention.

摘要

本文介绍了一种用于高灵敏度测量光动力疗法（PDT）和皮肤紫外线（UV）照射过程中产生的单线态氧发光的技术。通过使用计算光谱（CS）方法实现了高测量灵敏度，与光谱滤波方法相比，该方法提高了光子检测效率。使用固态铟镓砷光电二极管作为CS探测器，与光电倍增管相比，这显著降低了系统成本并提高了鲁棒性。光谱分辨率能够高精度地确定和扣除光敏剂荧光基线，而无需时间选通。这使得能够高灵敏度地检测由连续波光源（如太阳模拟器光源和PDT诊所常用的光源）产生的单线态氧发光发射。在体内和体外实验中证明了该技术的价值，这些实验表明测量的单线态氧与PDT治疗效果以及皮肤光照强度之间的相关性。这些结果证明了该技术作为剂量计指导PDT治疗以及作为支持开发用于预防皮肤癌的改进防晒产品的分析工具的潜在用途。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c40/10670281/c82628a85024/diagnostics-13-03431-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c40/10670281/a4b17111dbef/diagnostics-13-03431-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c40/10670281/2a71764a3484/diagnostics-13-03431-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c40/10670281/46567418f9d9/diagnostics-13-03431-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c40/10670281/bc0af65cdff3/diagnostics-13-03431-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c40/10670281/fbf62128d507/diagnostics-13-03431-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c40/10670281/4a8e836505fb/diagnostics-13-03431-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c40/10670281/57ad19e0a241/diagnostics-13-03431-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c40/10670281/c82628a85024/diagnostics-13-03431-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c40/10670281/a4b17111dbef/diagnostics-13-03431-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c40/10670281/2a71764a3484/diagnostics-13-03431-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c40/10670281/46567418f9d9/diagnostics-13-03431-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c40/10670281/bc0af65cdff3/diagnostics-13-03431-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c40/10670281/fbf62128d507/diagnostics-13-03431-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c40/10670281/4a8e836505fb/diagnostics-13-03431-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c40/10670281/57ad19e0a241/diagnostics-13-03431-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c40/10670281/c82628a85024/diagnostics-13-03431-g008.jpg

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3
Intracellular detection of singlet oxygen using fluorescent nanosensors.
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Analyst. 2021 Jun 14;146(12):3933-3941. doi: 10.1039/d1an00456e.
4
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J Biomed Opt. 2020 Mar;25(6):1-13. doi: 10.1117/1.JBO.25.6.063810.
5
Reactive Oxygen Species Explicit Dosimetry for Photofrin-mediated Pleural Photodynamic Therapy.活性氧种明确剂量测定在血啉介导的胸膜光动力疗法中的应用。
Photochem Photobiol. 2020 Mar;96(2):340-348. doi: 10.1111/php.13176. Epub 2019 Dec 6.
6
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7
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8
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