利用荧光纳米传感器检测细胞内的单线态氧。
Intracellular detection of singlet oxygen using fluorescent nanosensors.
机构信息
Department of Physics and Astronomy, University of Toledo, Toledo, Ohio, USA.
Department of Chemistry, University of Michigan, Ann Arbor, MI, USA.
出版信息
Analyst. 2021 Jun 14;146(12):3933-3941. doi: 10.1039/d1an00456e.
Detection of singlet oxygen is of great importance for a range of therapeutic applications, particularly photodynamic therapy, plasma therapy and also during photo-endosomolytic activity. Here we present a novel method of intracellular detection of singlet oxygen using biocompatible polymeric nanosensors, encapsulating the organic fluorescent dye, Singlet Oxygen Sensor Green (SOSG) within its hydrophobic core. The singlet oxygen detection efficiency of the nanosensors was quantified experimentally by treating them with a plasma source and these results were further validated by using Monte Carlo simulations. The change in fluorescence intensity of the nanosensors serves as a metric to detect singlet oxygen in the local micro-environment inside mammalian cancer cells. We used these nanosensors for monitoring singlet oxygen inside endosomes and lysosomes of cancer cells, during cold plasma therapy, using a room-temperature Helium plasma jet.
检测单线态氧对于一系列治疗应用非常重要，特别是光动力疗法、等离子体疗法，以及在光内溶酶体活性期间。在这里，我们提出了一种使用生物相容性聚合物纳米传感器在细胞内检测单线态氧的新方法，该方法将有机荧光染料单线态氧传感器绿（SOSG）封装在其疏水核内。通过用等离子体源处理纳米传感器，实验量化了纳米传感器的单线态氧检测效率，并且通过使用蒙特卡罗模拟进一步验证了这些结果。纳米传感器荧光强度的变化可作为在哺乳动物癌细胞内局部微环境中检测单线态氧的指标。我们使用这些纳米传感器在使用室温氦气等离子射流的冷等离子体治疗期间，在癌细胞的内体和溶酶体内部监测单线态氧。
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