NanoBioAnalysis Group-Department of Physical and Analytical Chemistry, University of Oviedo, Julián Clavería 8, 33006, Oviedo, Spain.
NanoBioAnalysis Group-Department of Physical and Analytical Chemistry, University of Oviedo, Julián Clavería 8, 33006, Oviedo, Spain; Biotechnology Institute of Asturias, University of Oviedo, Santiago Gascon Building, 33006, Oviedo, Spain.
Anal Chim Acta. 2022 Jun 15;1212:339658. doi: 10.1016/j.aca.2022.339658. Epub 2022 Feb 26.
Due to their immunotoxicity, aflatoxins are considered as very important natural contaminants of a wide variety of agricultural products. Although conventional techniques such as high-performance liquid chromatography (HPLC) and gas chromatography (GC) combined with mass spectrometry (MS) have traditionally been used to detect such food contaminants, they are relatively expensive, laborious, and time consuming which limits their use for in field analysis at the so-called point-of-care (POC). Electrochemical biosensors are emerging devices that meet these expectations since they rely in reliable, simple, inexpensive, portable, selective, and easy-to-use analytical procedures and instruments that can be used by unskilled personnel outside the laboratory. In this context, in this review article we summarize and provide authoritative opinion on the use of electrochemical biosensors for aflatoxins of interest for food control. Even though previous reviews have rightly covered this issue, the continuous research and improvements in this field, mostly related to the use of novel nanomaterials, make an update much needed. For this reason, this review covers the most relevant approaches reported in the period 2015-2021, focusing for the first time on the use of nanomaterials for improving the biosensors performance. The principles of the different strategies developed are discussed, and some examples of relevant approaches are highlighted, together with future prospects and challenges.
由于其免疫毒性，黄曲霉毒素被认为是各种农产品中非常重要的天然污染物。尽管传统技术，如高效液相色谱（HPLC）和气相色谱（GC）与质谱（MS）相结合，传统上一直用于检测此类食品污染物，但它们相对昂贵、繁琐且耗时，这限制了它们在所谓的即时护理（POC）现场分析中的使用。电化学生物传感器是新兴的设备，符合这些期望，因为它们依赖于可靠、简单、廉价、便携式、选择性和易于使用的分析程序和仪器，未经培训的人员在实验室外也可以使用。在这方面，在这篇综述文章中，我们总结并提供了关于电化学生物传感器用于食品控制中感兴趣的黄曲霉毒素的使用的权威意见。尽管之前的评论已经正确地涵盖了这个问题，但该领域的持续研究和改进，主要与新型纳米材料的使用有关，使得更新变得非常必要。因此，本综述涵盖了 2015-2021 年期间报道的最相关方法，首次重点关注纳米材料在提高生物传感器性能方面的应用。讨论了所开发的不同策略的原理，并突出了一些相关方法的例子，以及未来的前景和挑战。
