Qin Pengxiang, Ma Shangyuan, Li Changjin, Di Yanjiao, Liu Zihao, Wang Huiru, Li Yang, Jiang Shuzhen, Yang Weiren, Jiao Ning
Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China.
Toxics. 2023 Nov 3;11(11):902. doi: 10.3390/toxics11110902.
Bisphenol A (BPA), a kind of environmental toxin, widely impacts daily life. Cysteine (Cys) is a nutritionally important amino acid for piglets. However, it remains unclear whether Cys can alleviate BPA-induced oxidative damage in piglets. The aim of the present study was to explore the protective effects of Cys in BPA-challenged piglets. A total of twenty-four piglets were divided into four groups that were further subdivided based on the type of exposure (with or without 0.1% BPA) in a basal or Cys diet for a 28 d feeding trial. The results showed that BPA exposure decreased the piglets' average daily weight gain by 14.9%, and decreased dry matter, crude protein and ether extract digestibility by 3.3%, 4.5% and 2.3%, respectively; these decreases were attenuated by Cys supplementation. Additionally, Cys supplementation restored BPA-induced decreases in superoxide dismutase (SOD) and glutathione (GSH), and increases in malondialdehyde (MDA) levels, in the serum and jejunum ( < 0.05). Moreover, BPA decreased the jejunal mRNA expression of antioxidant genes, which were restored by Cys supplementation ( < 0.05). Cys also restored BPA and increased serum D-lactate levels and diamine oxidase (DAO) activity, and BPA decreased jejunal disaccharidase activity ( < 0.05). Further investigations in this study showed that the protective effects of Cys were associated with restoring intestinal barrier integrity by improving the jejunal morphology and enhancing the mRNA expression of tight junction proteins ( < 0.05). Collectively, the results herein demonstrated that Cys supplementation attenuated the impact of BPA-induced oxidative damage on growth performance, nutrient digestibility and intestinal function.
双酚A（BPA）是一种环境毒素，对日常生活有广泛影响。半胱氨酸（Cys）是仔猪营养上重要的氨基酸。然而，Cys是否能减轻BPA诱导的仔猪氧化损伤仍不清楚。本研究的目的是探讨Cys对受BPA挑战的仔猪的保护作用。将24头仔猪分为四组，根据暴露类型（添加或不添加0.1%BPA）在基础日粮或含Cys日粮中进行28天的饲养试验。结果表明，BPA暴露使仔猪平均日增重降低了14.9%，干物质、粗蛋白和乙醚提取物消化率分别降低了3.3%、4.5%和2.3%；补充Cys可减轻这些降低。此外，补充Cys可恢复BPA诱导的血清和空肠中超氧化物歧化酶（SOD）和谷胱甘肽（GSH）的降低以及丙二醛（MDA）水平的升高（P<0.05）。此外，BPA降低了空肠抗氧化基因的mRNA表达，补充Cys可使其恢复（P<0.05）。Cys还恢复了BPA诱导升高的血清D-乳酸水平和二胺氧化酶（DAO）活性，且BPA降低了空肠双糖酶活性（P<0.05）。本研究的进一步调查表明，Cys的保护作用与通过改善空肠形态和增强紧密连接蛋白的mRNA表达来恢复肠道屏障完整性有关（P<0.05）。总体而言，本文结果表明补充Cys可减轻BPA诱导的氧化损伤对生长性能、养分消化率和肠道功能的影响。
