Chen Fengming, Chen Jiayi, Chen Qinghua, Yang Lingyuan, Yin Jie, Li Yinghui, Huang Xingguo
College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China.
Hunan Co-Innovation Center of Animal Production Safety, CICAPS, Changsha 410128, China.
Antioxidants (Basel). 2021 Mar 16;10(3):468. doi: 10.3390/antiox10030468.
Oxidative stress is increasingly being recognized as a player in the pathogenesis of intestinal pathologies, and probiotics are becoming an attractive means of addressing it. The present study investigated the effects of dietary supplementation with (LAB) on intestinal integrity and oxidative damage in lipopolysaccharide (LPS)-challenged piglets. A total of 36 crossbred weaned piglets (Duroc × Landrace × Large Yorkshire) were randomly divided into three groups: (1) non-challenged controls (CON), (2) LPS-challenged controls (LPS), and (3) 0.2% LAB (2.01 × 10 CFU/g) + LPS treatment (LAB + LPS). On the 29th day of the experiment, the LPS and CON groups were injected intraperitoneally with LPS and saline at 100 ug/kg body weight, respectively. The results show that the LPS-induced elevation of the serum diamine oxidase (DAO) level and small intestinal crypt depth (CD) were reversed by the dietary addition of LAB, which also markedly increased the ileal expression of tight junction proteins (occludin, ZO-1, and claudin-1) in the LPS-challenged piglets. Furthermore, LAB supplementation normalized other LPS-induced changes, such as by decreasing malondialdehyde (MDA) in both the serum and intestinal mucosa and 8-hydroxy-2-deoxyguanosine (8-OHdG) in the jejunal mucosa, increasing glutathione reductase (GR) and glutathione peroxidase (GSH-Px) in both the serum and intestinal mucosa, and increasing glutathione (GSH) and superoxide dismutase (SOD) in the jejunal mucosa. LAB also activated Toll-like receptor (TLR)-Bruton's tyrosine kinase (Btk)-nuclear factor erythroid 2-related factor 2(Nrf2) signaling pathways in the intestine, suggesting that it plays a vital role in the ameliorative antioxidant capacity of weaned piglets. In summary, LAB increased intestinal integrity by improving the intestinal structure and tight junctions while enhancing antioxidant functions via the activation of the TLR-Btk-Nrf2 signaling pathway.
氧化应激在肠道疾病发病机制中的作用日益受到认可，益生菌正成为应对这一问题的有吸引力的手段。本研究调查了日粮添加乳酸菌（LAB）对脂多糖（LPS）攻击的仔猪肠道完整性和氧化损伤的影响。总共36头杂交断奶仔猪（杜洛克×长白×大白）被随机分为三组：（1）未受攻击的对照组（CON），（2）LPS攻击的对照组（LPS），和（3）0.2%LAB（2.01×10CFU/g）+LPS处理组（LAB+LPS）。在实验的第29天，LPS组和CON组分别以100μg/kg体重腹腔注射LPS和生理盐水。结果表明，日粮添加LAB可逆转LPS诱导的血清二胺氧化酶（DAO）水平升高和小肠隐窝深度（CD）增加，同时显著增加LPS攻击仔猪回肠紧密连接蛋白（闭合蛋白、ZO-1和claudin-1）的表达。此外，添加LAB使其他LPS诱导的变化恢复正常，如降低血清和肠黏膜中的丙二醛（MDA）以及空肠黏膜中的8-羟基-2-脱氧鸟苷（8-OHdG），增加血清和肠黏膜中的谷胱甘肽还原酶（GR）和谷胱甘肽过氧化物酶（GSH-Px），并增加空肠黏膜中的谷胱甘肽（GSH）和超氧化物歧化酶（SOD）。LAB还激活了肠道中的Toll样受体（TLR）-布鲁顿酪氨酸激酶（Btk）-核因子红细胞2相关因子2（Nrf2）信号通路，表明其在断奶仔猪抗氧化能力改善中起重要作用。总之，LAB通过改善肠道结构和紧密连接增加肠道完整性，同时通过激活TLR-Btk-Nrf2信号通路增强抗氧化功能。
