骨肉瘤细胞中小非编码 RNA 转录组的变化。
Changes in the small noncoding RNA transcriptome in osteosarcoma cells.
机构信息
Department of Orthopedic Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine (SJTUSM), Shanghai, People's Republic of China.
Department of Orthopaedics, The 72nd Group Army Hospital of PLA, Huzhou, 313000, Zhejiang, People's Republic of China.
出版信息
J Orthop Surg Res. 2023 Nov 24;18(1):898. doi: 10.1186/s13018-023-04362-8.
BACKGROUND
Osteosarcoma has the highest incidence among bone malignant tumors and mainly occurs in adolescents and the elderly, but the pathological mechanism is still unclear, which makes early diagnosis and treatment very difficult. Bone marrow mesenchymal stem cells (BMSCs) are considered to be one of the sources of osteosarcoma cells. Therefore, a full understanding of the gene expression differences between BMSCs and osteosarcoma cells is very important to explore the pathogenesis of osteosarcoma and facilitate the early diagnosis and treatment of osteosarcoma. Small noncoding RNAs (sncRNAs) are a class of RNAs that do not encode proteins but directly play biological functions at the RNA level. SncRNAs mainly include Piwi-interacting RNAs (piRNAs), small nucleolar RNAs (snoRNAs), small nuclear RNAs (snRNAs), repeat RNAs and microRNAs (miRNAs).
METHODS
In this study, we compared the expression of sncRNAs in BMSCs and osteosarcoma cells by high-throughput sequencing and qPCR and looked for differentially expressed sncRNAs. CCK-8, clone formation and transwell assay were used to detect the effect of sncRNA in MG63 cells.
RESULTS
We found that 66 piRNAs were significantly upregulated and 70 piRNAs were significantly downregulated in MG63 cells. As for snoRNAs, 71 snoRNAs were significantly upregulated and 117 snoRNAs were significantly downregulated in MG63 cells. As for snRNAs, 35 snRNAs were significantly upregulated and 17 snRNAs were significantly downregulated in MG63 cells. As for repeat RNAs, 6 repeat RNAs were significantly upregulated and 7 repeat RNAs were significantly downregulated in MG63 cells. As for miRNAs, 326 miRNAs were significantly upregulated and 281 miRNAs were significantly downregulated in MG63 cells. Overexpression of piRNA DQ596225, snoRNA ENST00000364830.2, snRNA ENST00000410533.1 and miRNA hsa-miR-369-5p inhibited the proliferation and migration of MG63 cells.
CONCLUSIONS
Our results provide a theoretical basis for the pathogenesis, early diagnosis and treatment of osteosarcoma.
背景
骨肉瘤是骨恶性肿瘤中发病率最高的一种，主要发生在青少年和老年人，但发病机制仍不清楚，这使得早期诊断和治疗非常困难。骨髓间充质干细胞（BMSCs）被认为是骨肉瘤细胞的来源之一。因此，充分了解 BMSCs 和骨肉瘤细胞之间的基因表达差异对于探讨骨肉瘤的发病机制和促进骨肉瘤的早期诊断和治疗非常重要。小非编码 RNA（sncRNAs）是一类不编码蛋白质但直接在 RNA 水平发挥生物学功能的 RNA。sncRNAs 主要包括 Piwi 相互作用 RNA（piRNAs）、小核仁 RNA（snoRNAs）、小核 RNA（snRNAs）、重复 RNA 和 microRNAs（miRNAs）。
方法
本研究通过高通量测序和 qPCR 比较了 BMSCs 和骨肉瘤细胞中 sncRNAs 的表达，并寻找差异表达的 sncRNAs。CCK-8、克隆形成和 Transwell 实验用于检测 sncRNA 在 MG63 细胞中的作用。
结果
我们发现，MG63 细胞中 66 个 piRNA 显著上调，70 个 piRNA 显著下调。snoRNA 中，MG63 细胞中 71 个 snoRNA 显著上调，117 个 snoRNA 显著下调。snRNA 中，MG63 细胞中有 35 个 snRNA 显著上调，17 个 snRNA 显著下调。重复 RNA 中，MG63 细胞中有 6 个重复 RNA 显著上调，7 个重复 RNA 显著下调。miRNA 中，MG63 细胞中有 326 个 miRNA 显著上调，281 个 miRNA 显著下调。piRNA DQ596225、snoRNA ENST00000364830.2、snRNA ENST00000410533.1 和 miRNA hsa-miR-369-5p 的过表达抑制了 MG63 细胞的增殖和迁移。
结论
本研究结果为骨肉瘤的发病机制、早期诊断和治疗提供了理论依据。
Zotero 插件